Inhibition by endomorphin-1 and endomorphin-2 of excitatory transmission in adult rat substantia gelatinosa neurons

Neuroscience
Tsugumi Fujita, Eiichi Kumamoto

Abstract

Intrathecally-administered endomorphin-1 and endomorphin-2 produce antinociceptive effects which are different from each other. In order to elucidate a cellular basis for this result, we examined the effects of endomorphin-1 and endomorphin-2 on holding currents and spontaneous glutamatergic excitatory transmission in substantia gelatinosa neurons of adult rat spinal cord slices by use of the whole-cell patch-clamp technique. In about half of the neurons examined, endomorphin-1 and endomorphin-2 produced an outward current having a similar amplitude (25-27 pA at 1 microM) at -70 mV with almost the same value of effective concentration producing half-maximal response (0.19-0.21 microM). Both of them reversed at a potential close to the equilibrium potential for K+, and had the slope conductance that was larger at negative (-120 to -140 mV) than positive potentials (-60 to -90 mV). The endomorphin-1 and endomorphin-2 currents were reduced in amplitude by K+-channel inhibitors, Ba2+ (100 microM) and 4-aminopyridine (1 mM), and also by mu-opioid receptor antagonist D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (1 microM) to a similar extent. The endomorphin-2 but not endomorphin-1 current amplitude was increased by dipeptidyl peptidase I...Continue Reading

References

Jul 1, 1985·The Journal of Physiology·R A North, J T Williams
May 26, 1986·Life Sciences·G W Pasternak, P J Wood
Aug 7, 1998·Brain Research. Developmental Brain Research·W RahmanA H Dickenson
Sep 25, 1998·Life Sciences·J P MathisG W Pasternak
Jan 8, 1999·Peptides·S Martin-SchildJ E Zadina
May 7, 1999·Brain Research Bulletin·S Fürst
Oct 3, 1999·Neuroscience Letters·C A WilliamsN J Dun
Jan 5, 2000·Life Sciences·G HorvathG Benedek
Jun 18, 2002·Journal of Neurochemistry·Dominique MassotteGraeme Milligan
Dec 6, 2002·British Journal of Pharmacology·Shinobu SakuradaTsukasa Sakurada
Oct 8, 2003·British Journal of Pharmacology·Su-Ying WuNae J Dun

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Citations

Jun 19, 2012·Medicinal Chemistry Research : an International Journal for Rapid Communications on Design and Mechanisms of Action of Biologically Active Agents·Renata PerlikowskaAnna Janecka
May 16, 2012·Anesthesia and Analgesia·Hiroyuki HondaTatsuro Kohno
Aug 23, 2008·Peptides·Anna JaneckaJakub Fichna
Oct 24, 2007·Peptides·Richard J Bodnar
Apr 11, 2012·Journal of Neuroscience Research·Kotaro MizutaEiichi Kumamoto
May 22, 2010·ChemMedChem·Attila KeresztesGéza Tóth
Oct 31, 2009·Basic & Clinical Pharmacology & Toxicology·Renata PerlikowskaAnna Janecka
Mar 3, 2007·Pharmacological Reviews·Jakub FichnaJean-Claude Do Rego

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