Inhibition by medroxyprogesterone acetate of interleukin-1β-induced collagen degradation by corneal fibroblasts

Investigative Ophthalmology & Visual Science
Hongyan ZhouKoh-Hei Sonoda

Abstract

To examine the effect of medroxyprogesterone 17-acetate (MPA) on interleukin-1β (IL-1β)-induced collagen degradation by corneal fibroblasts. Rabbit corneal fibroblasts were cultured in three-dimensional collagen gels with or without MPA. Collagen degradation was determined by measurement of hydroxyproline after acid hydrolysis. The expression or activity of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) was evaluated by immunoblot analysis or gelatin zymography. The phosphorylation of mitogen-activated protein kinases (MAPKs) in corneal fibroblasts was examined by immunoblot analysis. Cell proliferation and viability were evaluated by measurement of bromodeoxyuridine incorporation and the release of lactate dehydrogenase, respectively. MPA inhibited IL-1β-induced collagen degradation by corneal fibroblasts in a concentration- and time-dependent manner. MMP expression and activation as well as TIMP expression in corneal fibroblasts exposed to IL-1β were also inhibited by MPA. MPA had no effect on cell proliferation or viability. MPA inhibited the IL-1β-induced phosphorylation of p38 MAPK without affecting that of the MAPKs ERK or JNK. IL-1β-induced MMP expression and activation as well as col...Continue Reading

Citations

Aug 26, 2014·Immunopharmacology and Immunotoxicology·Jae-Wook YangIl-Whan Choi
Jun 19, 2016·Photochemistry and Photobiology·Taras ArdanJan Motlík
Oct 24, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella My CheungTrevor Sherwin
Feb 3, 2016·Clinical & Experimental Ophthalmology·Kazuhiro KimuraKoh-Hei Sonoda
Oct 7, 2020·The Ocular Surface·Raul E Ruiz-LozanoAlejandro Rodriguez-Garcia
Aug 28, 2021·International Journal of Molecular Sciences·Teruo NishidaJunko Murakami

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