PMID: 8938720Jan 1, 1996Paper

Inhibition by nystatin of Kv1.3 channels expressed in Chinese hamster ovary cells

Neuropharmacology
S J HahnL K Kaczmarek

Abstract

The patch-clamp technique was used to study the effects of nystatin on a cloned delayed rectifier potassium channel (Kv1.3) expressed in Chinese hamster ovary (CHO) cells. Kv1.3 currents recorded in the whole-cell configuration, using an intracellular solution containing nystatin, were subjected to a time- and concentration-dependent reduction in their amplitude and in the time constants of apparent inactivation. Direct application of nystatin to the cytoplasmic side of excised inside-out patches inhibited Kv1.3 currents and this inhibition was immediately reversible upon washout of the drug. In contrast, currents mediated by another delayed rectifier (Kv3.1) were not affected by this drug. The concentrations for nystatin and its structural analog, amphotericin B, required to produce half maximal inhibition (IC50) of the current were estimated to be about 3 and 60 microM, respectively. The effects of nystatin on the amplitude and inactivation of Kv1.3 currents were not voltage-dependent. In inside-out patches, tetraethylammonium (TEA) produced a rapid block of Kv1.3 currents upon the onset of a voltage pulse, while the inhibition by nystatin developed slowly. When co-applied with TEA, nystatin potentiated the extent of the TEA-...Continue Reading

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Citations

Nov 5, 2008·BMC Immunology·Fernanda Mello de QueirozGuilherme Suarez-Kurtz
Dec 5, 2015·Expert Opinion on Therapeutic Targets·Mireia Pérez-VerdaguerAntonio Felipe
Jan 30, 2004·Biotechnology and Bioengineering·Detmer SipkemaRené H Wijffels
Jan 12, 2008·Journal of Pharmacological Sciences·Min Ji SungBok Hee Choi
Jun 22, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xianpei WangDanna Tu
Jan 6, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyang Mi LeeBok Hee Choi
Jul 21, 2011·Journal of Immunotoxicology·Mohamed Ben SghaierLeila Chekir-Ghedira
Feb 22, 2007·The Journal of Pharmacology and Experimental Therapeutics·Sung Eun KimSang June Hahn

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