Inhibition effects on fermentation of hardwood extracted hemicelluloses by acetic acid and sodium

Bioresource Technology
Sara WaltonPeter van Walsum

Abstract

Extraction of hemicellulose from hardwood chips prior to pulping is a possible method for producing ethanol and acetic acid in an integrated forest bio-refinery, adding value to wood components normally relegated to boiler fuel. Hemicellulose was extracted from hardwood chips using green liquor, a pulping liquor intermediate consisting of aqueous NaOH, Na(2)CO(3), and Na(2)S, at 160 degrees C, held for 110 min in a 20 L rocking digester. The extracted liquor contained 3.7% solids and had a pH of 5.6. The organic content of the extracts was mainly xylo-oligosaccharides and acetic acid. Because it was dilute, the hemicellulose extract was concentrated by evaporation in a thin film evaporator. Concentrates from the evaporator reached levels of up to 10% solids. Inhibitors such as acetic acid and sodium were also concentrated by this method, presenting a challenge for the fermentation organisms. Fermentation experiments were conducted with Escherichia coli K011. The un-concentrated extract supported approximately 70% conversion of the initial sugars in 14 h. An extract evaporated down to 6% solids was also fermentable while a 10% solids extract was not initially fermentable. Strain conditioning was later found to enable fermentatio...Continue Reading

References

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Apr 2, 2009·Applied Biochemistry and Biotechnology·Byung-Hwan Um, G Peter van Walsum

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Citations

Apr 2, 2009·Applied Biochemistry and Biotechnology·Byung-Hwan Um, G Peter van Walsum
May 11, 2010·Journal of Industrial Microbiology & Biotechnology·Sara L WaltonG Peter van Walsum
Jan 20, 2010·Applied Biochemistry and Biotechnology·Byung-Hwan Um, G Peter van Walsum
Apr 16, 2010·Bioresource Technology·Byung-Hwan Um, G Peter van Walsum
Mar 10, 2015·Bioresource Technology·Shuangquan YaoChengrong Qin
Apr 28, 2012·Bioresource Technology·Ting-Hsiang LinShir-Ly Huang
Jan 13, 2017·Applied Biochemistry and Biotechnology·Rakhi Baddam, G Peter van Walsum
Mar 3, 2017·Biotechnology for Biofuels·Tooran KhazraiePedram Fatehi

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