Inhibition, Inactivation, and Recovery of Ammonia-Oxidizing Activity in Cometabolism of Trichloroethylene by Nitrosomonas europaea.

Applied and Environmental Microbiology
M R HymanD J Arp

Abstract

The kinetics of the cometabolism of trichloroethylene (TCE) by the ammonia-oxidizing soil bacterium Nitrosomonas europaea in short-term (<10-min) incubations were investigated. Three individual effects of TCE cometabolism on this bacterium were characterized. First, we observed that TCE is a potent competitive inhibitor of ammonia oxidation by N. europaea. The K(infi) value for TCE (30 (mu)M) is similar to the K(infm) for ammonia (40 (mu)M). Second, we examined the toxicity associated with TCE cometabolism by N. europaea. Stationary-phase cells of N. europaea oxidized approximately 60 nmol of TCE per mg of protein before ammonia-oxidizing activity was completely inactivated by reactive intermediates generated during TCE oxidation. At the TCE concentrations used in these experiments, ammonia did not provide significant protection against inactivation. Third, we have determined the ability of cells to recover ammonia-oxidizing activity after exposure to TCE. Cells recovering from TCE inactivation were compared with cells recovering from the specific inactivation of ammonia-oxidizing activity by light. The recovery kinetics were indistinguishable when 40% or less of the activity was inactivated. However, at increased levels of ina...Continue Reading

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Citations

Apr 25, 2001·Applied and Environmental Microbiology·C M YeagerD J Arp
Oct 31, 2002·Applied and Environmental Microbiology·Joonhong ParkLinda M Abriola
Feb 11, 2010·Applied Microbiology and Biotechnology·Luis A Sayavedra-SotoMark E Dolan
Dec 22, 2007·Archives of Microbiology·Sunhwa Park, Roger L Ely
Mar 21, 2007·Applied and Environmental Microbiology·Barbara O GvakhariaDaniel J Arp
Mar 29, 2000·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·E R Mitchell, M S Mayer
Feb 28, 2009·Applied Microbiology and Biotechnology·Barbara O GvakhariaLuis A Sayavedra-Soto
May 18, 2010·Applied and Environmental Microbiology·Oramas SuttinunEkawan Luepromchai
Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Apr 7, 2005·Applied and Environmental Microbiology·Daniel P ErwinRonald L Crawford
Jul 9, 2008·Applied and Environmental Microbiology·Sunhwa Park, Roger L Ely
Apr 6, 2017·Environmental Science and Pollution Research International·Iva DolinováAlena Ševců
Jul 2, 2008·Environmental Science & Technology·Tyler S RadnieckiLewis Semprini
Aug 24, 2018·Chemosphere·Miguel Martínez-JardinesFlor Cuervo-López
May 15, 2002·Environmental Pollution·M Cristina RomerAngélica M Arambarri
Nov 5, 1997·Applied and Environmental Microbiology·M E Fuller, K M Scow
Apr 27, 2012·Environmental Science & Technology·Christina L Arnaout, Claudia K Gunsch
Jan 14, 2004·Environmental Science & Technology·B TartakovskyS R Guiot

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