Inhibition of 125I organification and thyroid hormone release by interleukin-1, tumor necrosis factor-alpha, and interferon-gamma in human thyrocytes in suspension culture

The Journal of Clinical Endocrinology and Metabolism
K SatoY Ito

Abstract

To elucidate the mechanism of decreased 131I uptake by the thyroid gland in patients with subacute thyroiditis and painless thyroiditis, human thyroid follicles were cultured with interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF alpha), and/or interferon-gamma (IFN gamma), and the effects of these cytokines on thyroid function were studied in vitro. When human thyrocytes were cultured in RPMI-1640 medium containing 0.5% fetal calf serum and TSH for 5-8 days, the cells incorporated 125I, synthesized de novo [125I]iodotyrosines and [125I]iodothyronines, and secreted [125I]T4 and [125I]T3 into the medium. IL-1 alpha and IL-1 beta inhibited 125I incorporation and [125I]iodothyronine release in a concentration-dependent manner. The minimal inhibitory effect was detected at 10 pg/ml. Electron microscopic examination revealed a marked decrease in lysosome formation in IL-1-treated thyrocytes. TNF alpha and IFN gamma also inhibited thyroid function in a concentration-dependent manner. Furthermore, when thyrocytes were cultured with IL-1, TNF alpha and IFN gamma, these cytokines more than additively inhibited thyroid function. Although the main mechanism of 131I uptake suppression in the thyroid gland in subacute thyroiditis is du...Continue Reading

Citations

Apr 9, 2013·Endocrine·Sun hee KimHeung Yong Jin
Jul 16, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y HirookaT Mitsuma
May 16, 1997·Molecular and Cellular Endocrinology·P H DaviesJ A Franklyn
Oct 24, 1998·Domestic Animal Endocrinology·R L MatteriR W Johnson
Mar 4, 2000·Domestic Animal Endocrinology·S KahlJ W Blum
Apr 9, 1998·Baillière's Clinical Endocrinology and Metabolism·A P WeetmanP F Watson
Jan 7, 1999·Clinical Endocrinology·R A AjjanA P Weetman
Aug 24, 2002·Thyroid : Official Journal of the American Thyroid Association·Polyxeni KoutkiaRobert M Levin
Apr 18, 2003·Thyroid : Official Journal of the American Thyroid Association·Kazuko YamazakiKanji Sato
Jan 11, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E P CorssmitJ A Romijn
Apr 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·H HashimotoT Sato
Oct 1, 1996·Thyroid : Official Journal of the American Thyroid Association·R KanriI Makino
Aug 1, 1997·Thyroid : Official Journal of the American Thyroid Association·A E PekaryJ M Hershman
Feb 26, 2000·Thyroid : Official Journal of the American Thyroid Association·J de MetzH P Sauerwein
Aug 21, 2008·Thyroid : Official Journal of the American Thyroid Association·Andrew G GianoukakisTerry J Smith
Dec 23, 2009·Thyroid : Official Journal of the American Thyroid Association·Kazuko YamazakiKanji Sato
May 18, 2010·Thyroid : Official Journal of the American Thyroid Association·Yuji Nagayama
Oct 2, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nicole C KleinstreuerRichard S Judson
Sep 1, 1996·Medicine·L C Hofbauer, A E Heufelder
Apr 1, 1992·Clinical Endocrinology·A P Weetman
Dec 5, 2008·Journal of Gastroenterology and Hepatology·Khaleel M JamilWendy Cheng
May 1, 1997·Journal of Pediatric Endocrinology & Metabolism : JPEM·J Balter-Seri, S Ashkenazi
Feb 27, 2003·American Journal of Veterinary Research·David L PancieraDaniel L Ward
Jun 19, 2013·Indian Journal of Endocrinology and Metabolism·Zohreh Jadali
Oct 1, 2013·Thyroid : Official Journal of the American Thyroid Association·Ides M ColinAnne-Catherine Gérard
May 15, 2015·The Journal of Endocrinology·Emmely M de VriesAnita Boelen
May 21, 2016·BioMed Research International·Shujing JiNanping Wu
Nov 7, 2007·Endocrinology and Metabolism Clinics of North America·Yaron TomerNagako Akeno
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Frank B Diamond, Duane C Eichler
Mar 25, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jamie C MandacYaron Tomer

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