Inhibition of 36Cl uptake by stilbene sulphonic acid derivatives and loop diuretics in rat submandibular salivary acini.

Archives of Oral Biology
J R Martinez, S Barker

Abstract

Acini were isolated from submandibular glands by enzymatic digestion and incubated in HCO3-containing and HCO3-free [hydroxyethyl-1-piperazine-ethane sulphonic acid (HEPES) buffered] solutions to compare the accumulation of the isotopic tracer 36Cl in the absence and presence of stilbene sulphonic acid derivatives and of loop diuretics. Tracer accumulation was similar in both solutions in the absence of inhibitors and reached 17-19 nmol/mg protein after 5 min of incubation. Exposure to 10(-3) M 4,4'-diisothiocyano-2,2'-stilbene disulphonic acid (DIDS) or to 10(-4) M bumetanide in HCO3-buffered medium resulted in significant reductions in both the initial phase (from 0 to 5 min) and the steady-state phase (5-30 min) of 36Cl accumulation. By contrast, in HCO3-free, HEPES-buffered incubation solution, DIDS was significantly less effective in reducing 36Cl accumulation, while bumetanide was somewhat more effective than in HCO3-buffered medium. At a higher dose (10(-3) M), furosemide was less effective than bumetanide in reducing the steady-state tracer content of the cells in the HCO3-buffered medium (22% versus 35% reduction), although the effects were similar in the initial phase of tracer uptake. Exposure to 10(-4) M 4-acetamido...Continue Reading

References

Aug 1, 1986·The American Journal of Physiology·B Nauntofte, J H Poulsen
Feb 1, 1987·Pflügers Archiv : European journal of physiology·D PiraniJ A Young
Feb 1, 1987·Journal of Dental Research·J A YoungD Pirani
Mar 1, 1988·Journal of Dental Research·J R Martinez, P Reed
Jan 1, 1988·Archives of Oral Biology·J R MartinezP Reed
Dec 1, 1986·Pflügers Archiv : European journal of physiology·J R Martinez, N Cassity
Nov 1, 1987·The American Journal of Physiology·M MontroseG A Kimmich
May 1, 1986·Pflügers Archiv : European journal of physiology·L H SmajeH H Ussing
Dec 1, 1986·Pflügers Archiv : European journal of physiology·I Novak, J A Young
Jan 1, 1985·Pflügers Archiv : European journal of physiology·J R Martinez, N Cassity
Feb 23, 1984·Nature·O H Petersen, Y Maruyama

❮ Previous
Next ❯

Citations

Jun 1, 1994·Journal of Cellular Physiology·J C SeagraveJ R Martinez
Apr 1, 1992·Archives of Oral Biology·S Barker, J R Martinez
Nov 1, 1996·Advances in Dental Research·K S Prostak, Z Skobe
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J R Martinez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.