PMID: 6968407Sep 25, 1980

Inhibition of 40S--Met--tRNAfMet ribosomal initiation complex formation by vaccinia virus

A PersonG Beaud


Infection with vaccinia virus (a poxvirus) quickly and efficiently shuts off host protein synthesis in the presence of actinomycin D (refs 3--5) or cycloheximide. The cellular messenger RNA apparently remains stable in the infected cells exposed to inhibitors of viral gene transcription. In some cases vaccinia viral RNA or poly(A) synthesis have been implicated in the establishment of this effect. However, in the presence of cordycepin (3-deoxyadenosine) which blocks viral gene transcription and cytoplasmic poly(A) synthesis, cellular protein synthesis is still efficiently inhibited in vaccinia virus-infected cells. This shutoff is also observed in vitro, in the corresponding cell-free extracts, and in a reticulocyte lysate. Therefore the shutoff of host protein synthesis is probably mediated by a factor associated with vaccinia virions. We now report that the formation of the 40S--Met-tRNAfMet initiation complex is inhibited in cytoplasmic extracts derived from vaccinia virus-infected cells exposed to cordycepin to block viral gene expression. A similar inhibition is found in reticulocyte lysates incubated with purified vaccinia cores, confirming the hypothesis that the factor associated with the viral cores is responsible for...Continue Reading


Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Ben-Hamida, G Beaud
Dec 1, 1977·The Australian and New Zealand Journal of Psychiatry·M Girgis
Jan 1, 1978·Annual Review of Biochemistry·M Revel, Y Groner
Jan 1, 1979·Annual Review of Biochemistry·S Ochoa, C de Haro
Jan 1, 1976·Annual Review of Biochemistry·H F Lodish
May 1, 1973·The Journal of General Virology·M Esteban, D H Metz


Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D HörleinP Bornstein
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G Coppola, R Bablanian
Mar 21, 1983·FEBS Letters·P W Tas, O H Martini
Jun 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng QinDengXue Lu
Jan 1, 1992·Archives of Virology·C R Dâmaso, N Moussatché

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