Inhibition of ACh-stimulated exocytosis by NSAIDs in guinea pig antral mucous cells: autocrine regulation of mucin secretion by PGE2

American Journal of Physiology. Gastrointestinal and Liver Physiology
Chikao ShimamotoTakashi Nakahari

Abstract

The effects of indomethacin (IDM) and aspirin (ASA) on ACh (10 microM) -stimulated exocytotic events were studied in guinea pig antral mucous cells by using video optical microscopy. IDM or ASA, which inhibits cyclooxygenase (COX), decreased the frequency of ACh-stimulated exocytotic events by 30% or 60%, respectively. The extent of inhibition induced by ASA (60%) decreased by 30% when IDM or arachidonic acid (AA, the substrate of COX) was added. IDM, unlike ASA, appears to induce the accumulation of AA, which enhances the frequency of ACh-stimulated exocytotic events in ASA-treated cells. ONO-8713 (100 microM; an inhibitor of the EP1-EP4 prostaglandin receptors) and N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, HCl (H-89, 20 microM; an inhibitor of PKA) also decreased the frequency of ACh-stimulated exocytotic events by 60%. However, the supplementation of PGE(2) (1 microM) prevented the IDM-induced decrease in the frequency of ACh-stimulated exocytotic events. SC-560 (an inhibitor of COX-1) decreased the frequency of ACh-stimulated exocytotic events by 30%, but NS-398 (an inhibitor of COX-2) did not. Moreover, IDM decreased the frequency of exocytotic events stimulated by ionomycin, suggesting that COX-1 acti...Continue Reading

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Citations

May 25, 2010·The Journal of Physiological Sciences : JPS·Hideyo YoshidaTakashi Nakahari
Jan 31, 2016·Experimental Parasitology·Luana Aparecida CossentiniPhileno Pinge-Filho
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adel H SaadYoshinori Marunaka
Mar 2, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Saori TanakaTakashi Nakahari
Aug 4, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikao ShimamotoTakashi Nakahari
Oct 25, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Saori TanakaTakashi Nakahari

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