PMID: 7526840Oct 15, 1994Paper

Inhibition of adenylate cyclase activity by galanin in rat insulinoma cells is mediated by the G-protein Gi3

The Biochemical Journal
P de MazancourtL S Weinstein

Abstract

Galanin inhibits adenylate cyclase activity and insulin secretion and modulates ion channels in pancreatic beta-cells through pertussis-toxin-sensitive G-protein(s). Antibodies directed against the C-terminal region of specific G-protein alpha-subunits were used to determine which G-protein(s) couple galanin receptors to inhibition of adenylate cyclase in the rat insulinoma cell line RINm5F. Preincubation of membranes with EC antibody (anti-alpha i3) decreased the inhibition of forskolin-stimulated adenylate cyclase activity by galanin (100 nM) by 45% compared with control IgG (P < 0.05) whereas preincubation with AS (anti-alpha i1, alpha i2) or GO (anti-alpha o) antibodies had no significant effect. To confirm these results, RINm5F cells were exposed intermittently over a 4-day period to phosphorothioate oligodeoxynucleotides that were either sense or antisense to alpha i1, alpha i2, alpha i3 or alpha o. Oligodeoxynucleotides antisense to alpha i2, alpha i3 and alpha o specifically decreased the levels of the targeted alpha-subunit in membranes. alpha i1 was undetectable in these cells. Inhibition of adenylate cyclase activity by galanin was largely abolished in membranes from cells exposed to the oligodeoxynucleotide antisens...Continue Reading

Citations

Dec 26, 1996·Annals of the New York Academy of Sciences·J C MarieG Skoglund
Oct 2, 1998·British Journal of Pharmacology·N NiiroH Kanaide
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Guanghua TangMeisheng Jiang
Nov 14, 1998·The American Journal of Physiology·C A HirshmanC W Emala
Dec 1, 1995·Cellular and Molecular Neurobiology·K KaskT Bartfai
Dec 1, 1996·The American Journal of Physiology·G W Sharp
Dec 28, 1995·Brain Research. Molecular Brain Research·E M ParkerM E Goldstein
Sep 22, 1995·Regulatory Peptides·J N Crawley
Mar 8, 2002·Cellular Signalling·Paul R Albert, Liliane Robillard
Apr 3, 1999·Neuropeptides·E Karelson, U Langel

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