PMID: 8604255Feb 1, 1996Paper

Inhibition of adherence of Actinomyces naeslundii (Actinomyces viscosus) T14V-J1 to saliva-treated hydroxyapatite by a monoclonal antibody to type 1 fimbriae

Oral Microbiology and Immunology
W E NesbittW B Clark

Abstract

A monoclonal antibody to Actinomyces naeslundii (A. viscosus) T14V-J1 type 1 fimbriae, capable of inhibiting the adherence of this bacterium to salivary proline-rich protein-treated hydroxyapatite, was generated by immunization of SWR mice with A. naeslundii 55-19, a strain derived from T14V-J1 that possess only type 1 fimbriae. Supernatants of hybridomas were screened for reactivity with purified type 1 fimbriae. An IgG monoclonal antibody, 86-49E, blocked the adsorption of the parent strain to proline-rich protein-treated hydroxyapatite by 77% with 1.0 microgram/ml of the monoclonal antibody; the Fab fragment derived from this monoclonal antibody inhibited adherence by 38% at the same concentration. Similarly, the adherence of strain 55-19 was inhibited by 100% and 64% to proline-rich protein-treated hydroxyapatite with 1.0 micrograms/ml of IgG and Fab fragments respectively. Control monoclonal antibody to the subunit of type 1 fimbriae, as well as to Actinobacillus actinomycetemcomitans caused only minimal adherence inhibition. Monoclonal antibody 86-49E also agglutinated both type 1 fimbriae-bearing strains of A. naeslundii T14V-J1 and 55-19 but not strains 59-51 and 147, which lack type 1 fimbriae. Further confirmation of ...Continue Reading

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Citations

May 3, 2011·Journal of Bacteriology·Chenggang WuHung Ton-That
Feb 6, 2007·Journal of Bacteriology·Arunima MishraHung Ton-That
Feb 22, 2008·Gerodontology·Sergio Sánchez-GarcíaCarmen García-Peña
Dec 18, 2007·Trends in Microbiology·Anjali MandlikHung Ton-That
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·W F Liljemark, C Bloomquist
Dec 15, 2010·Applied and Environmental Microbiology·Stefan RuhlHelmut Durchschlag
Mar 27, 1999·Oral Microbiology and Immunology·S HamadaI Morisaki
Jan 14, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·N Takahashi, T Yamada

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