Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARgamma2

Journal of Cell Science
Yoshiyuki TanabeKoichi Nakayama

Abstract

This study investigated the effects of cyclic stretching on adipocyte differentiation of mouse preadipocyte 3T3-L1 cells. Confluent 3T3-L1 cells were treated with dexamethasone, 3-isobutyl-1-methylxanthine and insulin for 45 hours (induction period), followed by incubation with insulin for 9 additional days (maturation period). A transient burst of CCAAT/enhancer-binding protein (C/EBP) beta and C/EBPdelta at an early stage (approximately 3 hours) and a delayed induction (approximately 45 hours) of C/EBPalpha and PPARgamma(2) were sequentially provoked during the induction period. Application of cyclic stretching during the entire induction period or only during the final 15 hours of the induction period significantly retarded the induction of glycerol-3-phosphate dehydrogenase (GPDH) activity and the accumulation of intracellular triglycerides by the end of the maturation period. Cyclic stretching for the entire induction period, as well as that applied during the final 15 hours of the induction period, significantly reduced the expression of PPARgamma(2) mRNA, whereas reduction in the expression of C/EBPdelta mRNA was only observed in response to stretching that had been applied during the entire induction period. The express...Continue Reading

References

May 15, 1994·Genes & Development·P TontonozB M Spiegelman
Nov 1, 1993·Cell Transplantation·K Kanda, T Matsuda
Jan 1, 1997·Annual Review of Physiology·J Sadoshima, S Izumo
Jul 23, 1998·Physiological Reviews·F M GregoireH S Sul
Aug 23, 2000·The Journal of Clinical Investigation·B B Kahn, J S Flier
Sep 30, 2000·Journal of Vascular Surgery·N AzumaB E Sumpio
Jan 21, 2001·The Journal of Biological Chemistry·S W KimH S Ro
Mar 10, 2001·Cell·B M Spiegelman, J S Flier
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Mar 7, 2002·American Journal of Physiology. Cell Physiology·F Machinal-QuélinY Giudicelli
Aug 2, 2002·Cell·Benjamin Geiger, Alexander Bershadsky
Oct 2, 2002·Pflügers Archiv : European journal of physiology·Kristina J CsuklyPhillip F Gardiner
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Alexandra D OldenhofStephen J Lye
Dec 7, 2002·Hypertension·James R Sowers, Steven Haffner
Apr 23, 2003·European Journal of Pharmacology·Maki SaitoKoichi Nakayama
Jun 13, 2003·Nature·Toshimasa YamauchiTakashi Kadowaki

❮ Previous
Next ❯

Citations

Jan 29, 2010·Cellular and Molecular Life Sciences : CMLS·Edwin C M Mariman, Ping Wang
Jan 12, 2012·Biomechanics and Modeling in Mechanobiology·Naama Shoham, Amit Gefen
Dec 29, 2011·Annals of Biomedical Engineering·Ayelet LevyAmit Gefen
Oct 28, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·E J BakJ-H Cha
Dec 7, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·V L VeumG Mellgren
Apr 7, 2007·Critical Care Medicine·Michael G VishBasilia Zingarelli
Feb 5, 2011·Clinical and Experimental Pharmacology & Physiology·Hong-Yan LingDuan-Fang Liao
Feb 2, 2011·Journal of Biomechanical Engineering·Markus RottmarKatharina Maniura-Weber
Mar 15, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ranjit S ChimaBasilia Zingarelli
Sep 30, 2006·American Journal of Respiratory Cell and Molecular Biology·Srinivas PapaiahgariSekhar P Reddy
Sep 26, 2007·Head & Face Medicine·Paul Svedman
Aug 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Koichi Nakayama
Oct 27, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yoshiyuki Tanabe, Koichi Nakayama
Dec 18, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ganesan MuraliViswanathan Saraswathi
Mar 27, 2010·Journal of Endocrinological Investigation·S SunC Wang
Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Tomoji OnoHoyoku Nishino
Oct 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·C T RubinS Judex
Sep 26, 2012·Annual Review of Nutrition·Gabriel Martinez-Santibañez, Carey Nien-Kai Lumeng
Sep 12, 2014·The Journal of Endocrinology·M van de VyverW F Ferris
Dec 3, 2014·Journal of Biomechanics·Yousef ShafieyanThomas M Quinn
Nov 27, 2009·Cellular and Molecular Life Sciences : CMLS·Jongsung LeeDeokhoon Park
Mar 3, 2012·Differentiation; Research in Biological Diversity·Ghazaleh KhayatThomas M Quinn
Jan 24, 2012·Biochemical and Biophysical Research Communications·Jeong Soon LeeJung Yul Lim
Feb 20, 2016·Plastic and Reconstructive Surgery. Global Open·Yi YuanRei Ogawa
Jul 6, 2010·Molecular and Cellular Endocrinology·H Sadie-Van GijsenW F Ferris
Feb 18, 2010·Journal of Bioscience and Bioengineering·Md Golam HossainKatsuhiko Yoshimoto
Sep 16, 2008·Molecular and Cellular Endocrinology·Sang Dal RheeHyae Gyeong Cheon
Mar 11, 2008·Metabolism: Clinical and Experimental·Vanessa A ConstantAlexander Sorisky
May 21, 2008·International Journal of Cosmetic Science·A V Rawlings
Mar 8, 2012·Journal of Cellular Biochemistry·Jongsung LeeDeokhoon Park
Apr 6, 2016·Frontiers in Bioengineering and Biotechnology·Ya-Ju ChangChia-Ching Wu
Jun 11, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Shada AbuhattumDaphne Weihs

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.