Inhibition of ADP-induced platelet aggregation by apoE is not mediated by membrane cholesterol depletion

Thrombosis Research
D R Riddell, J S Owen

Abstract

We have previously shown that plasma HDL-E, a minor subclass of high-density lipoproteins (HDL) containing apolipoprotein (apo) E, has a potent anti-platelet effect and implicated apoE as the active constituent. Recently, apoE complexes with phospholipids (DMPC) were reported to inhibit thrombin-induced aggregation by sequestering platelet membrane cholesterol. Here we demonstrate that platelet cholesterol depletion is an improbable explanation for the suppressive effect of apoE:DMPC on ADP-mediated platelet aggregation; only 0.5% of cholesterol was released prior to addition of ADP to initiate aggregation while lactoferrin, which does not accept cellular cholesterol, was also inhibitory. Previous studies have shown that apoE and lactoferrin are both bound by platelets but whether this provides the initial stimulus for suppression of aggregation remains to be established.

References

Mar 1, 1975·The Journal of Clinical Investigation·S J ShattilR A Cooper
Nov 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J PedreñoF González-Sastre
Dec 31, 1991·Biochemical and Biophysical Research Communications·M HigashiharaK Kurokawa
Apr 22, 1991·FEBS Letters·M HigashiharaK Kurokawa
May 22, 1990·Biochimica Et Biophysica Acta·S ParthasarathyL G Fong
Jan 1, 1986·Methods in Enzymology·J C Osborne
Jan 1, 1986·Methods in Enzymology·S C RallR W Mahley
Jul 6, 1984·Biochimica Et Biophysica Acta·D E Newton, J M Shaw
Jan 1, 1981·Annals of Nutrition & Metabolism·D R IllingworthP Alaupovic
Jan 3, 1980·The New England Journal of Medicine·M J StuartJ G White
Dec 1, 1994·Current Opinion in Lipidology·A von EckardsteinG Assmann
Apr 1, 1995·Current Opinion in Lipidology·H Jingami, T Yamamoto
Jun 1, 1994·Current Opinion in Lipidology·E R Skinner
Aug 1, 1994·The Journal of Clinical Investigation·C PásztyE M Rubin
Nov 15, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·H M WilsonE R Skinner
Jan 1, 1994·Advances in Protein Chemistry·K H Weisgraber
May 1, 1993·The International Journal of Biochemistry·A ManevaL Sirakov

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Citations

Apr 26, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Vandana S DoleMonty Krieger
Apr 6, 2005·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Małgorzata Bednarska-MakarukHanna Wehr

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