Inhibition of Al(III)-Induced Aβ42 Fibrillation and Reduction of Neurotoxicity by Epigallocatechin-3-Gallate Nanoparticles

Journal of Biomedical Nanotechnology
Neha Atulkumar SinghZaved Ahmed Khan

Abstract

Accumulation of amyloid beta fibrils is the pathological hallmark of Alzheimer's disease. Epigallocatechin-3-gallate (EGCG) has shown to possess potent anti-amyloidogenic, metal chelation and antioxidant properties. However, its therapeutic potential is limited in-vivo due to its poor bioavailability and stability. Therefore, the present study aims to evaluate the neuroprotective role of EGCG nanoparticles (nanoEGCG) against Al(III)-induced Aβ42 fibrillation in-vitro. NanoEGCG was synthesized and its physiochemical characterization was performed. In-vitro release profiles and stability of nanoEGCG in simulated gastro-intestinal fluids, along with its antioxidant and metal chelation potential was evaluated. The anti-amyloidogenic potential of nanoEGCG on Aβ42 secondary structure and its morphology was evaluated via induction with Al(III) and nanoEGCG treatment. Further, the effect of Aβ42 on cellular toxicity was also assessed. NanoEGCG with 96% encapsulation efficiency and a hydrodynamic diameter of 300 nm with spherical to slightly ellipsoid shape was synthesized. EGCG release from the nanoparticle occurred in a sustained manner and was stable when released in simulated gastro-intestinal fluids. The antioxidant and metal chela...Continue Reading

Citations

Jan 21, 2020·Expert Review of Neurotherapeutics·Ewa Baranowska-Wójcik, Dominik Szwajgier
Nov 12, 2020·Biological Trace Element Research·Muhammad Faisal IkramTouqeer Ahmed
Feb 15, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yilan XuHeng Zhang
Oct 5, 2021·Critical Reviews in Food Science and Nutrition·Ramkumar SamynathanJosé M Lorenzo

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