PMID: 6991324Mar 1, 1980Paper

Inhibition of alloxan action in isolated pancreatic islets by superoxide dismutase, catalase, and a metal chelator

Diabetes
L J Fischer, S A Hamburger

Abstract

The possible participation of superoxide anions, hydrogen peroxide, and hydroxyl radicals in the action of alloxan was investigated using isolated rat pancreatic islets. Exposure of islets for 5 min to alloxan (0.15 or 0.2 mg/ml) inhibited subsequent glucose-stimulated insulin release. The presence of superoxide dismutase (1000 U/ml), catalase (50 microgram/ml), or a metal chelator diethylenetriaminepentacetic acid (1 mM) markedly attenuated this effect of alloxan. Use of these agents afforded complete protection from the lower concentration of alloxan and partial protection from the higher concentration of the toxic compound. Inactivation of the enzymes or addition of excess iron to the chelating agent before its use with alloxan eliminated the protective action of these agents. The results are consistent with the proposal that hydroxyl radicals, generated via reactions that involve superoxide anions, hydrogen peroxide, and iron, mediate the deleterious effect of alloxan in pancreatic islets.

Citations

Dec 1, 1984·Diabetologia·G L WilsonB T Mossman
Dec 1, 1989·Die Naturwissenschaften·M NukatsukaJ Kawada
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·R NorlundI B Täljedal
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·L Jansson, S Sandler
Jun 1, 1986·Journal of Neurocytology·L G ThaeteS S Spicer
Jul 1, 1981·Diabetologia·I B Täljedal
Jun 27, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Koichi SakuraiYukio Fujimoto
Oct 15, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·S L Marklund, B Hägglöf
Jan 1, 1986·The International Journal of Biochemistry·K Grankvist, S L Marklund
Jan 1, 1988·The International Journal of Biochemistry·J Z Byczkowski, T Gessner
Jan 1, 1989·The International Journal of Biochemistry·K Grankvist
Dec 19, 1983·Life Sciences·K Grankvist, S L Marklund
Jan 1, 1988·Life Sciences·T Miura, K Sakurai
Mar 1, 1985·Pharmacology, Biochemistry, and Behavior·J M Murnane, S Ritter
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B O Izokun-EtiobhioE N Ugochukwu
Nov 1, 1992·Toxicology Letters·M S Abdel-RahmanF A Iskander
Dec 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P YadavS Sarkar
Jan 1, 1988·Free Radical Biology & Medicine·L W Oberley
Jan 1, 1988·Free Radical Biology & Medicine·C Richter, B Frei
Jan 1, 1992·Free Radical Biology & Medicine·D W Reif
Apr 1, 1993·Free Radical Biology & Medicine·J G CorneliusA B Peck
Feb 8, 2000·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M A El-Missiry
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·D L EizirikM E Hoffmann
Sep 19, 1998·Free Radical Biology & Medicine·M MurataS Kawanishi
Jun 11, 1981·The New England Journal of Medicine·G F Cahill, H O McDevitt
Jun 18, 1981·The New England Journal of Medicine·S E GandyG M Galbraith
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W J MalaisseD G Pipeleers
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H M KubischJ P Phillips
Jan 1, 1986·Journal of the American College of Nutrition·P A Crapo
Nov 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H ZhangU T Brunk
Apr 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H ZhangU T Brunk
Jan 7, 2005·Journal of Medical Genetics·R BergholdtF Pociot
Sep 1, 1982·The Journal of Clinical Investigation·S E GandyR K Crouch

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.