PMID: 6403034Jan 20, 1983Paper

Inhibition of amino acid incorporation in a cell-free system and inhibition of protein synthesis in cultured cells by reaction products of selenite and thiols

Biochimica Et Biophysica Acta
L N VernieW S Bont

Abstract

Reaction products of selenite with thiols were tested for an inhibitory effect on amino acid incorporation in a cell-free system derived from rat liver and on protein synthesis in intact P815 and L1210 cells. In the cell-free system maximum inhibition, up to 96%, was reached at about 10 microM selenium. In intact cells inhibitory effect varied depending on which reaction product or cell line was used. Maximum inhibition was obtained after 30 min of incubation with selenium concentrations ranging from 0.25 microM to over 7 microM. Selenite itself also inhibited protein synthesis of L1210 cells, but only after 90 min of incubation and starting at selenium concentrations of 2 microM. Inhibition of protein synthesis in intact cells was followed by cell death. Pre-incubation of the reaction products of a monothiol (2-propanethiol) and of a vicinal dithiol (2,3-dimercapto-1-propanol) in culture medium showed a rapid decrease of the inhibitory capability of the product from the monothiol, but not of the product from the dithiol. The results indicate that selenite and a thiol react to form products which have differential toxic effects to cells in vitro.

References

Jan 1, 1979·Advances in Cancer Research·A C Griffin
Apr 15, 1979·The Biochemical Journal·L N VernieI T Wilders
Jan 15, 1974·Biochemistry·L N VernieP Emmelot
Jan 15, 1961·Biochimica Et Biophysica Acta·G A EVERETT, R W HOLLEY
Mar 1, 1979·Biological Trace Element Research·K A Poirier, J A Milner

❮ Previous
Next ❯

Citations

Nov 1, 1993·Biological Trace Element Research·C MacVicar, G D Frenkel
Nov 1, 1992·Biological Trace Element Research·P B Caffrey, G D Frenkel
Apr 1, 1989·Biological Trace Element Research·J A Golczewski, G D Frenkel
Jul 1, 1991·Biological Trace Element Research·G D FrenkelC MacVicar
May 7, 1998·Cancer Letters·P B Caffrey, G D Frenkel
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H MatsumuraO Hayaishi
Jun 4, 2002·Reviews on Environmental Health·M VincetiG Vivoli
Jan 1, 1992·Leukemia Research·X R JiangA C Newland
Mar 7, 2002·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Claudio CermelliMarinella Portolani
Feb 18, 2014·Basic & Clinical Pharmacology & Toxicology·Marita WallenbergMikael Björnstedt
Feb 1, 1986·The Journal of Dermatology·K HanadaY Katabira
Dec 12, 2012·The Science of the Total Environment·Marco VincetiSaverio Stranges
May 1, 1986·Arthritis and Rheumatism·X WeiL Sokoloff
Aug 15, 1991·Archives of Biochemistry and Biophysics·F IslamO Hayaishi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.