Inhibition of amino acid transport by sphingoid long chain bases in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
M S SkrzypekR C Dickson

Abstract

Sphingoid long chain bases have many effects on cells including inhibition or stimulation of growth. The physiological significance of these effects is unknown in most cases. To begin to understand how these compounds inhibit growth, we have studied Saccharomyces cerevisiae cells. Growth of tryptophan (Trp-) auxotrophs was more strongly inhibited by phytosphingosine (PHS) than was growth of Trp+ strains, suggesting that PHS diminishes tryptophan uptake and starves cells for this amino acid. This hypothesis is supported by data showing that growth inhibition is relieved by increasing concentrations of tryptophan in the culture medium and by multiple copies of the TAT2 gene, encoding a high affinity tryptophan transporter. Measurement of tryptophan uptake shows that it is inhibited by PHS. Finally, PHS treatment induces the general control response, indicating starvation for amino acids. Multiple copies of TAT2 do not protect cells against two other cationic lipids, stearylamine, and sphingosine, indicating that the effect of PHS on tryptophan utilization is specific. Other data demonstrate that PHS reduces uptake of leucine, histidine, and proline by specific transporters. Our data suggest that PHS targets proteins in the amino ...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jan 1, 1991·Methods in Enzymology·F Sherman, J Hicks
May 1, 1990·Biochimica Et Biophysica Acta·A H Merrill, D D Jones
Jan 1, 1985·Advances in Microbial Physiology·J M WiameH N Arst
Dec 22, 1986·Biochimica Et Biophysica Acta·J Horák
Apr 25, 1986·Cell·P P Mueller, A G Hinnebusch
Nov 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S SteinerR L Lester
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S A Johnston, J E Hopper
Apr 1, 1983·Molecular and Cellular Biology·W E Courchesne, B Magasanik
Aug 1, 1993·Molecular and Cellular Biology·J HeitmanM N Hall
Oct 1, 1994·Trends in Biochemical Sciences·A G Hinnebusch
Jan 1, 1995·FEMS Microbiology Reviews·V Sophianopoulou, G Diallinas
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Oct 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Spiegel, A H Merrill
Apr 1, 1997·Archives of Biochemistry and Biophysics·K M EllaK E Meier
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M BartschA R Subramanian

❮ Previous
Next ❯

Citations

Dec 2, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Schneiter
Jul 2, 2003·Archives of Biochemistry and Biophysics·Brooke S WrightDaniel V Lynch
Jun 19, 2002·Biochimica Et Biophysica Acta·Robert C Dickson, Robert L Lester
Jan 18, 2003·Biochimica Et Biophysica Acta·Lina M ObeidCungui Mao
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sumin HanAmy Chang
Sep 10, 2010·The Journal of Biological Chemistry·Soraya S JohnsonW Scott Moye-Rowley
Apr 2, 2011·Cold Spring Harbor Perspectives in Biology·J Thomas HannichHoward Riezman
Aug 11, 2004·Eukaryotic Cell·Marcin KolaczkowskiW Scott Moye-Rowley
Oct 12, 2000·Molecular and Cellular Biology·F Abe, K Horikoshi
Dec 17, 2002·Molecular and Cellular Biology·Jijun ChengXiang S Ye
Nov 3, 2011·The Journal of Clinical Investigation·Benjamin T Bikman, Scott A Summers
Nov 2, 2007·Theoretical Biology & Medical Modelling·Fernando Alvarez-VasquezYusuf A Hannun
Feb 10, 2012·PLoS Genetics·Xinhe HuangRobert C Dickson
Nov 19, 2009·Biological Chemistry·Enno C I VeermanArie V Nieuw Amerongen
Nov 14, 2013·Chemistry and Physics of Lipids·David J MontefuscoYusuf A Hannun
Jan 5, 2013·Biochimica Et Biophysica Acta·Robert L LesterRobert C Dickson
Nov 17, 2007·Journal of Applied Microbiology·D MormeneoA Delgado
Feb 15, 2007·FEMS Microbiology Reviews·Jaime AguileraJose Antonio Prieto
May 7, 2009·FEMS Yeast Research·Myriam Manente, Michel Ghislain
Nov 8, 2013·Molecular Biology of the Cell·Erwin SwinnenJoris Winderickx
May 16, 2002·The Journal of Biological Chemistry·Alfred H Merrill
Jun 1, 2018·Journal of Cell Science·Brendan T FinicleAimee L Edinger
Mar 1, 2015·Eukaryotic Cell·Svetlana KhakhinaW Scott Moye-Rowley
Nov 16, 2007·Organic & Biomolecular Chemistry·David MormeneoAntonio Delgado
Jan 13, 2006·The Journal of Biological Chemistry·Sneh Lata Panwar, W Scott Moye-Rowley
Jun 19, 2003·The Journal of Biological Chemistry·Carlos J Rodriguez-HernandezJosé R Murguia
Dec 22, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Russell HydeHarinder S Hundal

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.