Inhibition of amyloid fibrillation of human γD-crystallin by gold nanoparticles: Studies at molecular level

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Vandna SharmaKalyan Sundar Ghosh

Abstract

The capability of citrate-stabilized gold nanoparticles (AuNps) has been explored for the inhibition of amyloid fibrillation of human γD-crystallin (HGD), a major protein of eye lens. Citrate-capped AuNps were synthesized, characterized and used further for amyloid inhibition. The results from intrinsic and extrinsic (in the presence of Thioflavin T and ANS) fluorescence based assays and CD spectroscopy clearly suggest that AuNps at nanomolar concentrations can act as an effective inhibitor against fibrillation of HGD. Fluorescence microscopic and transmission electron microscopic images also supported this observation. Considering the inhibitory role of AuNps against HGD fibrillation, interactions between HGD and AuNps were studied to decipher the mechanism of amyloid inhibition. The binding and quenching constants were calculated as ~109 M-1 using the data of tryptophan fluorescence quenching of HGD by AuNps. Ground state complexation between the protein and nanoparticles was predicted. AuNps were not found to cause any major conformational changes in the native protein. Entropy-driven complexation process between the protein and nanoparticles indicates the interactions of AuNps with hydrophobic residues of HGD. Therefore, in...Continue Reading

Citations

May 30, 2020·Journal of Biomolecular Structure & Dynamics·Shiwani Rana, Kalyan Sundar Ghosh
Dec 22, 2020·International Journal of Biological Macromolecules·Malak Abu-HussienDaniel Segal
Sep 11, 2021·Journal of Molecular Recognition : JMR·Pooja Prasanthan, Nand Kishore

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