Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP.

Journal of Nanobiotechnology
Fadee G MondalekRama Ramanujam

Abstract

The redox dye, DCPIP, has recently shown to exhibit anti-melanoma activity in vitro and in vivo. On the other hand, there is increasing evidence that synthetic nanoparticles can serve as highly efficient carriers of drugs and vaccines for treatment of various diseases. These nanoparticles have shown to serve as potent tools that can increase the bioavailability of the drug/vaccine by facilitating absorption or conferring sustained and improved release. Here, we describe results on the effects of free- and nanoparticle-enclosed DCPIP as anti-angiogenesis and anti-inflammation agents in a human colon cancer HCT116 cell line in vitro, and in induced angiogenesis in ovo. The studies described in this report indicate that (a) DCPIP inhibits proliferation of HCT116 cells in vitro; (b) DCPIP can selectively downregulate expression of the pro-angiogenesis growth factor, VEGF; (c) DCPIP inhibits activation of the transcriptional nuclear factor, NF-kappaB; (d) DCPIP can attenuate or completely inhibit VEGF-induced angiogenesis in the chick chorioallantoic membrane; (e) DCPIP at concentrations higher than 6 mug/ml induces apoptosis in HCT116 cells as confirmed by detection of caspase-3 and PARP degradation; and (f) DCPIP encapsulated in n...Continue Reading

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Citations

Sep 6, 2011·Molecular Cancer Therapeutics·Dharmalingam SubramaniamShrikant Anant
Feb 10, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Luc R A RougéeAbby C Collier
Aug 22, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pingting ZhuZhaoguo Liu

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Methods Mentioned

BETA
Assay
electrophoresis
Protein Assay

Software Mentioned

Prism

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