Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2

Proceedings of the National Academy of Sciences of the United States of America
Melanie BolteStefan Irniger

Abstract

Proteolysis triggered by the anaphase-promoting complex (APC) is needed for sister chromatid separation and the exit from mitosis. APC is a ubiquitin ligase whose activity is tightly controlled during the cell cycle. To identify factors involved in the regulation of APC-mediated proteolysis, a Saccharomyces cerevisiae GAL-cDNA library was screened for genes whose overexpression prevented degradation of an APC target protein, the mitotic cyclin Clb2. Genes encoding G1, S, and mitotic cyclins were identified, consistent with previous data showing that the cyclin-dependent kinase Cdk1 associated with different cyclins is a key factor for inhibiting APC(Cdh1) activity from late-G1 phase until mitosis. In addition, the meiosis-specific protein kinase Ime2 was identified as a negative regulator of APC-mediated proteolysis. Ectopic expression of IME2 in G1 arrested cells inhibited the degradation of mitotic cyclins and of other APC substrates. IME2 expression resulted in the phosphorylation of Cdh1 in G1 cells, indicating that Ime2 and Cdk1 regulate APC(Cdh1) in a similar manner. The expression of IME2 in cycling cells inhibited bud formation and caused cells to arrest in mitosis. We show further that Ime2 itself is an unstable protei...Continue Reading

References

Sep 1, 1992·Genes & Development·C B Epstein, F R Cross
Jan 1, 1991·Methods in Enzymology·J R PringleB K Haarer
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Aug 22, 1997·Cell·M SchwabW Seufert
Oct 23, 1998·Science·S ChuI Herskowitz
Jan 23, 1999·Current Opinion in Cell Biology·J M Peters
Mar 11, 1999·Current Opinion in Genetics & Development·A Amon
Aug 31, 1999·Genes & Development·W Zachariae, K Nasmyth
Nov 13, 1999·Nature Cell Biology·D O Morgan
Dec 22, 1999·Current Opinion in Cell Biology·W Zachariae
Dec 23, 1999·Current Opinion in Microbiology·P Jorgensen, M Tyers
Jun 28, 2000·The Journal of Cell Biology·A D RudnerA W Murray
Sep 7, 2000·Cell·M A Hoyt
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·K F CooperR Strich
Jul 11, 2001·Novartis Foundation Symposium·K NasmythF Uhlmann
Nov 8, 2001·Current Opinion in Cell Biology·B Lee, A Amon

❮ Previous
Next ❯

Citations

Sep 2, 2006·Nature Cell Biology·Marie-Emilie Terret, Prasad V Jallepalli
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Adèle L Marston, Angelika Amon
Jun 5, 2003·Genes & Development·Kirsten R BenjaminIra Herskowitz
Sep 30, 2010·Molecular and Cellular Biology·Natalie StrudwickMartin Schröder
Dec 22, 2010·Molecular and Cellular Biology·Yi-Ju LaiTing-Fang Wang
Dec 17, 2004·Molecular and Cellular Biology·Kedar PurnapatreSaul M Honigberg
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuhiro SuzumoriMartin M Matzuk
Feb 26, 2004·Molecular Microbiology·Patrick DieckhoffStefan Irniger
Feb 11, 2011·Molecular Microbiology·Stefan Irniger
Jun 11, 2011·FEMS Microbiology Reviews·Ozgür Bayram, Gerhard H Braus
Nov 30, 2010·Fungal Genetics and Biology : FG & B·Kuang-Hung Liu, Wei-Chiang Shen
Jul 20, 2004·Journal of Cellular Biochemistry·Saul M Honigberg
Jul 29, 2015·Molecular and Cellular Biology·Liang JinAaron M Neiman
Nov 25, 2003·The Journal of Biological Chemistry·Dawn M CliffordGeorge S Brush
Mar 1, 2003·The Journal of Biological Chemistry·Mark C HallChristoph H Borchers
May 11, 2006·The Journal of Biological Chemistry·Karen Schindler, Edward Winter
Nov 25, 2004·Journal of Cell Science·Heng-Yu ChangKeith T Jones
Sep 5, 2002·Genes & Development·J Wade HarperMark J Solomon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.