Inhibition of apical domain formation does not block blastocyst development in bovine embryos.

Reproduction, Fertility, and Development
S A A Dos AnjosM D Goissis

Abstract

The first event of cellular differentiation consists of the segregation of the trophectoderm and the inner cell mass. Studies in mice suggest that cell contractility and the formation of an apical domain play important roles in this event; however, this remains unknown in the bovine. We tested the hypothesis that blocking apical domain formation would halt subsequent trophectoderm differentiation in bovine embryos. We first assessed the formation of an apical domain by the presence of Par-6 Family Cell Polarity Regulator Beta (PARD6B) and Ezrin (EZR), which appeared after the 8-cell stage. We inhibited apical domain formation by blocking cell contractility with 25μM (-)-blebbistatin. Treatment from 90 to 186h after insemination did not reduce blastocyst development compared with the untreated control group or the group treated with inactive (+)-blebbistatin. Immunofluorescence staining after blebbistatin treatment revealed the absence of EZR and the trophectoderm marker Caudal Type Homeobox 2 (CDX2). Following blebbistatin treatment, Yes1 Associated Transcriptional Regulator (YAP), which is involved in the Hippo signalling pathway, exhibited cytoplasmic staining instead of nuclear localisation. Despite changes in protein expres...Continue Reading

References

Jun 1, 1988·Biology of Reproduction·J J ParrishN L First
Feb 1, 1983·Biology of Reproduction·B D BavisterG Lieberman
Jan 1, 1994·Biology of Reproduction·H KoyamaR H Foote
Jan 20, 2005·Journal of Cell Science·Berenika PlusaMagdalena Zernicka-Goetz
Dec 18, 2007·Mechanisms of Development·Noriyuki NishiokaHiroshi Sasaki
Aug 31, 2010·Cytoskeleton·Mutsuki AmanoKozo Kaibuchi
Feb 15, 2011·Developmental Cell·Debra K BergPeter L Pfeffer
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jun 25, 2013·Current Biology : CB·Yoshikazu HirateHiroshi Sasaki
Sep 25, 2014·Molecular Reproduction and Development·Marcelo D Goissis, Jose B Cibelli
Sep 27, 2014·Current Opinion in Cell Biology·Pedro Gaspar, Nicolas Tapon
Oct 14, 2014·Developmental Cell·David M BryantKeith E Mostov
Dec 13, 2016·The Journal of Reproduction and Development·Nobuyuki SakuraiKen Sawai
Feb 9, 2017·Theriogenology·Regivaldo Vieira de SousaMaurício Machaim Franco
Feb 9, 2017·Developmental Cell·Ekaterina KorotkevichTakashi Hiiragi
Oct 17, 2017·Nature Communications·Meng ZhuMagdalena Zernicka-Goetz
Mar 16, 2018·Molecular Reproduction and Development·Verónica M Negrón-PérezPeter J Hansen
Mar 27, 2018·Cell·Jennifer ZenkerNicolas Plachta
Apr 18, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroki AkizawaManabu Kawahara

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