Abstract
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a highly toxic persistent organic pollutant. Most of the toxic effects of TCDD are believed to be mediated by high-affinity binding to the aryl hydrocarbon receptor (AhR) and subsequent effects on gene transcription and protein expression. TCDD causes cancer in multiple tissues in different animal species and is classified as a class 1 human carcinogen. In initiation-promotion studies, TCDD was shown to be a potent liver-tumor promotor. Among other theories it has been hypothesized that TCDD promotes tumor growth by preventing initiated cells from correctly executing apoptosis. In this study, we examined the effects of TCDD on apoptosis induced by UV-C light, ochratoxin A (OTA), and cycloheximide (CHX) in primary rat hepatocytes. Both UV-C light and OTA caused caspase activation and nuclear apoptotic effects. CHX did not activate caspases but nevertheless caused DNA fragmentation and chromatin condensation. TCDD inhibited UV-C light-induced apoptosis and this effect seemed to be dependent on AhR-activation as was shown by employing an AhR antagonist. In contrast to UV-C light-induced apoptosis, TCDD failed to protect primary rat hepatocytes from OTA- or CHX-induced apoptosis. Since ...Continue Reading
References
Jan 1, 1976·Methods in Cell Biology·P O Seglen
Nov 1, 1978·Toxicology and Applied Pharmacology·R J KocibaC G Humiston
May 7, 1976·Analytical Biochemistry·M M Bradford
Mar 1, 1992·Carcinogenesis·D SchrenkK W Bock
Aug 1, 1972·British Journal of Cancer·J F KerrA R Currie
Jun 1, 1995·Carcinogenesis·S StinchcombeM Schwarz
Mar 1, 1994·Carcinogenesis·D SchrenkK W Bock
Nov 22, 1997·FEBS Letters·A V AlessenkoE D Makarieva
May 26, 1998·Environmental Health Perspectives·D B McGregorJ M Rice
Jul 25, 1998·HNO·M C Jäckel
Dec 11, 1999·Biochemical Pharmacology·W M BlomJ F Nagelkerke
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Oct 2, 2004·Carcinogenesis·Gerd PääjärviJohan Högberg
Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Kumiko Samejima, William C Earnshaw
Oct 11, 2005·Toxicology·Sujin Park, Fumio Matsumura
Oct 28, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·M Marin-KuanB Schilter
Sep 15, 2006·The Journal of Pharmacology and Experimental Therapeutics·Jingxiang Bai, Arthur I Cederbaum
Sep 16, 2006·Molecular Nutrition & Food Research·Stefanie Knerr, Dieter Schrenk
May 8, 2007·Archives of Biochemistry and Biophysics·Kaname Kawajiri, Yoshiaki Fujii-Kuriyama
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gopa BiswasNarayan G Avadhani
Jul 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·Maricel Marin-KuanBenoît Schilter
Oct 7, 2008·Toxicology·Chayma BouazizHassen Bacha
Jun 13, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Martin ChopraDieter Schrenk
Citations
Jun 14, 2014·PloS One·Rong-Jane ChenYing-Jan Wang
Apr 5, 2011·Toxicology·Robert RoosDieter Schrenk
Sep 28, 2015·The Science of the Total Environment·Markus BrinkmannHenner Hollert
Jul 7, 2015·Regulatory Toxicology and Pharmacology : RTP·Richard A BeckerRobert A Budinsky
May 3, 2012·Biochemistry Research International·Karine AndreauAida Bouharrour
Nov 20, 2013·Critical Reviews in Toxicology·R A BudinskyJ C Rowlands
Jan 23, 2015·Toxicology Mechanisms and Methods·Radhakrishna SuraB Bhaskar Gollapudi
Sep 7, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Helena Ufelmann, Dieter Schrenk
Sep 18, 2012·Aquatic Toxicology·Martine H GjernesAugustine Arukwe
Feb 18, 2011·Critical Reviews in Toxicology·Martin Chopra, Dieter Schrenk
Feb 9, 2012·Toxicology and Industrial Health·Hasan TurkezMokhtar I Yousef
Jul 11, 2012·Toxicology and Industrial Health·Hasan TurkezZuhal Polat
Oct 29, 2011·Toxicology and Industrial Health·Hasan TürkezMokhtar I Yousef