Inhibition of apoptosis in serum starved porcine embryonic fibroblasts

Molecular Reproduction and Development
Chang-Kyu Lee, Jorge A Piedrahita

Abstract

In nuclear transplantation, serum starvation is a general method to synchronize donor cells at the quiescent stage (G(0)) of the cell cycle. However, serum starvation during culture of mammalian cells may induce cell death, especially through apoptosis, thus contributing to the low efficiency of nuclear transplantation. This study was performed to characterize apoptosis during serum starvation and to determine the effects of apoptosis inhibitors such as a protease inhibitor [alpha(2)-macroglobulin (MAC)] and antioxidants [N-acetylcysteine (NAC), glutathione (GSH)] on serum starved porcine embryonic fibroblasts (PEF). PEF, collected from day 25-30 porcine fetuses, were cultured for 5 days in media containing 0.5% FBS to induce quiescence. Serum starved PEF showed typical morphology of apoptotic cells and stained for DNA fragmentation by TUNEL assay (26.7%). All apoptosis inhibitors tested in this study significantly (P < 0.05) reduced apoptosis of serum starved PEF, with antioxidants having better results (MAC: 7.4% vs. NAC: 1.0%, and GSH: 0.8%). Equally and importantly, the treatment with apoptosis inhibitors did not change the proportion of G(0)/G(1) stage cells. Therefore, the addition of MAC and antioxidants during serum sta...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Sep 1, 1989·Biology of Reproduction·R S PratherN L First
Sep 1, 1971·The Journal of Pathology·J F Kerr
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J LotemL Sachs
Jan 1, 1996·Advances in Experimental Medicine and Biology·J J Cohen
Feb 27, 1997·Nature·I WilmutK H Campbell
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Dec 16, 1998·Science·Y KatoY Tsunoda
Mar 20, 1999·Biology of Reproduction·A C BoquestR S Prather
May 20, 1999·Nature Biotechnology·A BaguisiY Echelard

❮ Previous
Next ❯

Citations

Nov 16, 2012·BMC Cell Biology·Helen A FosterJoanna M Bridger
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan
Dec 18, 2013·Acta Veterinaria Hungarica·Manita WittayaratTakeshige Otoi
May 30, 2003·Biology of Reproduction·Martha C GómezCharles Earle Pope
Jun 5, 2003·Molecular Reproduction and Development·Martin AngerHeiner Niemann
Dec 6, 2006·The Journal of Veterinary Medical Science·Byoung-Chol OhWoo-Suk Hwang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis