Inhibition of astrocytes promotes long-distance growing nerve fibers in ventral mesencephalic cultures

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
Sara Af BjerkénIngrid Strömberg

Abstract

Tyrosine hydroxylase-positive nerve fiber formation occurs in two diverse morphological patterns in rat fetal ventral mesencephalic slice cultures; one is non-glial-associated and the other is glial-associated. The aim of this study was to characterize the non-glial-associated nerve fibers and its relation to migration of astrocytes. Organotypic slice cultures were prepared from embryonic days 12, 14, and 18 rat fetuses and maintained for 5, 7 or 14 days in vitro. Inhibition of cell proliferation using cytosine beta-D-arabinofuranoside was conducted in embryonic day 14 ventral mesencephalic cultures. The treatment impaired astrocytic migration at 7 and 14 days in vitro. The reduced migration of astrocytes exerted a negative effect on the glial-associated tyrosine hydroxylase-positive nerve fibers, reducing the outgrowth from the tissue slice. The non-glial-associated outgrowth was, however, positively affected by reduced astrocytic migration, reaching distances around 3mm in 2 weeks, and remained for longer time in culture. Co-cultures of fetal ventral mesencephalon and frontal cortex revealed the cortex as a target for the non-glial-associated tyrosine hydroxylase-positive outgrowth. The age of the fetal tissue at plating affe...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Jul 15, 1964·Experientia·A Dahlström, K Fuxe
May 26, 1995·Brain Research. Developmental Brain Research·H KawanoI Nagatsu
Nov 5, 1997·Journal of Neurochemistry·P P MichelY Agid
Nov 5, 1997·Trends in Neurosciences·B H GähwilerS M Thompson
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T HaradaK Tanaka
Feb 20, 1999·Archives of Neurology·R A HauserC W Olanow
Jan 3, 2001·Developmental Biology·E HartfussM Götz
Mar 10, 2001·The New England Journal of Medicine·C R FreedS Fahn
Jul 14, 2001·The New England Journal of Medicine·D E RedmondD D Spencer
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lars M BjorklundOle Isacson
Jan 25, 2002·The Journal of Comparative Neurology·Saga Johansson, Ingrid Strömberg
Mar 16, 2002·Nature Neuroscience·Nico HeinsMagdalena Götz
Jan 28, 2003·Biochemical Society Transactions·A Hidalgo
Mar 12, 2003·Neuron·Paolo MalatestaMagdalena Götz
Dec 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Florian T MerkleArturo Alvarez-Buylla
Jan 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathalie SpasskyArturo Alvarez-Buylla
Jan 25, 2007·The Journal of Comparative Neurology·Elisabet BerglöfIngrid Strömberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.

Astrocytes in Parkinson Disease

Parkinson's disease (PD) is a neurodegenerative disorder caused by the progressive loss of dopaminergic neurons. Some PD-genes may be associated with astrocyte dysfunction. Discover the latest research on astrocytes in Parkinson's disease here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.