Inhibition of autophagy impairs tumor cell invasion in an organotypic model.

Cell Cycle
Robin L MacintoshKevin M Ryan

Abstract

Autophagy is a membrane-trafficking process that delivers cytoplasmic constituents to lysosomes for degradation. It contributes to energy and organelle homeostasis and the preservation of proteome and genome integrity. Although a role in cancer is unquestionable, there are conflicting reports that autophagy can be both oncogenic and tumor suppressive, perhaps indicating that autophagy has different roles at different stages of tumor development. In this report, we address the role of autophagy in a critical stage of cancer progression-tumor cell invasion. Using a glioma cell line containing an inducible shRNA that targets the essential autophagy gene Atg12, we show that autophagy inhibition does not affect cell viability, proliferation or migration but significantly reduces cellular invasion in a 3D organotypic model. These data indicate that autophagy may play a critical role in the benign to malignant transition that is also central to the initiation of metastasis.

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Citations

Jun 19, 2013·Seminars in Cancer Biology·Robin L Macintosh, Kevin M Ryan
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Methods Mentioned

BETA
flow cytometry
gene knockdown
transfection
FCS

Software Mentioned

Image J
ImageJ

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