Inhibition of autophagy in EBV-positive Burkitt's lymphoma cells enhances EBV lytic genes expression and replication

Cell Death & Disease
Alessandra De LeoElena Mattia

Abstract

Autophagy, an important degradation system involved in maintaining cellular homeostasis, serves also to eliminate pathogens and process their fragments for presentation to the immune system. Several viruses have been shown to interact with the host autophagic machinery to suppress or make use of this cellular catabolic pathway to enhance their survival and replication. Epstein Barr virus (EBV) is a γ-herpes virus associated with a number of malignancies of epithelial and lymphoid origin in which establishes a predominantly latent infection. Latent EBV can periodically reactivate to produce infectious particles that allow the virus to spread and can lead to the death of the infected cell. In this study, we analyzed the relationship between autophagy and EBV reactivation in Burkitt's lymphoma cells. By monitoring autophagy markers and EBV lytic genes expression, we demonstrate that autophagy is enhanced in the early phases of EBV lytic activation but decreases thereafter concomitantly with increased levels of EBV lytic proteins. In a cell line defective for late antigens expression, we found an inverse correlation between EBV early antigens expression and autophagosomes formation, suggesting that early after activation, the virus...Continue Reading

References

Jan 15, 1984·International Journal of Cancer. Journal International Du Cancer·K Takada
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dai Iwakiri, Kenzo Takada
Jan 30, 2004·Journal of Virology·Wen-hai FengShannon C Kenney
Sep 24, 2005·Cell·Sophie PattingreBeth Levine
Oct 13, 2007·Infectious Agents and Cancer·Stefania MattiussiElena Mattia
Nov 13, 2007·Autophagy·Chengyu LiangJae U Jung
Nov 17, 2007·Cell Host & Microbe·Anthony OrvedahlBeth Levine
Mar 21, 2008·Virology·Ying-Ray LeeHsiao-Sheng Liu
Mar 22, 2008·AIDS·Dejiang Zhou, Stephen A Spector
Jul 4, 2008·Journal of Virology·Jerry WongHonglin Luo
Sep 2, 2008·Autophagy·Daniel J KlionskyDavid C Rubinsztein
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marlène DreuxFrancis V Chisari
Jan 25, 2011·Nature Cell Biology·Joungmok KimKun-Liang Guan
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Dec 30, 2011·Journal of Virology·Magali ChaumorcelAudrey Esclatine
May 3, 2014·Microbiology and Immunology·Takayuki Murata
Jun 4, 2014·Virology·Stephanie J BouleyAisling S Dugan

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Citations

Sep 27, 2016·Trends in Immunology·Christian Münz
Dec 7, 2017·Viruses·Marion Lussignol, Audrey Esclatine
Mar 21, 2018·Nature Reviews. Microbiology·Younho ChoiJae U Jung
Jan 31, 2018·International Journal of Hematology·Han Zhang
Aug 22, 2017·Archives of Virology·Jieying PuXi Huang
Dec 19, 2016·Apoptosis : an International Journal on Programmed Cell Death·Peiqi WangLixia Chen
Dec 1, 2019·Viruses·Zhouwulin ShaoVincent Maréchal
Apr 18, 2018·Journal of Cellular Physiology·Kiana Hosseinpour-MoghaddamAmirhossein Sahebkar
Mar 16, 2019·Frontiers in Oncology·Kwai Fung HuiAlan Kwok Shing Chiang
Jun 20, 2018·International Journal of Molecular Sciences·Domenico MattoscioSusanna Chiocca
Jul 2, 2019·Journal of Molecular Biology·Sebastian E Koschade, Christian H Brandts
Mar 26, 2021·Frontiers in Oncology·Alejandra SuaresOmar Coso
Apr 27, 2021·Molecular Therapy. Nucleic Acids·Javid Sadri NahandHamed Mirzaei
Nov 24, 2017·Journal of Virology·Qiming LiangJae U Jung

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Methods Mentioned

BETA
PCR
FCS
Protein Assay

Software Mentioned

GraphPad
ImageJ
GraphPad Prism6

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