Inhibition of Axon Regeneration by Liquid-like TIAR-2 Granules

Neuron
Matthew G AndrusiakYishi Jin

Abstract

Phase separation into liquid-like compartments is an emerging property of proteins containing prion-like domains (PrLDs), yet the in vivo roles of phase separation remain poorly understood. TIA proteins contain a C-terminal PrLD, and mutations in the PrLD are associated with several diseases. Here, we show that the C. elegans TIAR-2/TIA protein functions cell autonomously to inhibit axon regeneration. TIAR-2 undergoes liquid-liquid phase separation in vitro and forms granules with liquid-like properties in vivo. Axon injury induces a transient increase in TIAR-2 granule number. The PrLD is necessary and sufficient for granule formation and inhibiting regeneration. Tyrosine residues within the PrLD are important for granule formation and inhibition of regeneration. TIAR-2 is also serine phosphorylated in vivo. Non-phosphorylatable TIAR-2 variants do not form granules and are unable to inhibit axon regeneration. Our data demonstrate an in vivo function for phase-separated TIAR-2 and identify features critical for its function in axon regeneration.

References

May 1, 1974·Genetics·S Brenner
Mar 19, 2003·The Journal of Cell Biology·Helene TourrièreJamal Tazi
Dec 17, 2004·Nature·Mehmet Fatih YanikAdela Ben-Yakar
Sep 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zilu WuAndrew D Chisholm
Oct 28, 2008·Nature Genetics·Christian Frøkjaer-JensenErik M Jorgensen
Dec 9, 2008·Molecular Cell·Tobias M Franks, Jens Lykke-Andersen
Jan 24, 2009·Science·Marc HammarlundMichael Bastiani
Feb 6, 2009·Journal of Cell Science·María Gabriela ThomasGraciela L Boccaccio
Jan 13, 2010·Molecular Cell·J Ross Buchan, Roy Parker
Oct 8, 2011·Genes & Development·Christian Kroun Damgaard, Jens Lykke-Andersen
Feb 16, 2012·Nature Reviews. Neuroscience·Frank BradkeMicha E Spira
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Feb 13, 2013·Annals of Neurology·Peter HackmanBjarne Udd
Aug 6, 2013·Genesis : the Journal of Genetics and Development·Carlos Giovanni Silva-García, Rosa Estela Navarro
Jan 10, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Paola NixMichael Bastiani
Jan 17, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas HubertYishi Jin
May 6, 2014·Current Opinion in Neurobiology·Marc Hammarlund, Yishi Jin
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Shuya HuangJunying Miao
Jun 4, 2015·Cell Cycle·Carmen Sánchez-Jiménez, José M Izquierdo
Feb 13, 2016·G3 : Genes - Genomes - Genetics·Gabriela Huelgas-MoralesRosa E Navarro
Mar 10, 2016·Scientific Reports·Oleg O GlebovJuan Burrone
May 7, 2016·Neuron·Zhigang He, Yishi Jin
Oct 8, 2016·Methods : a Companion to Methods in Enzymology·Jean-Yves TinevezKevin W Eliceiri
Dec 24, 2016·Neuron·Sung Min HanMarc Hammarlund
Jan 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yiqing LiLarry Benowitz

❮ Previous
Next ❯

Citations

Feb 12, 2020·Medicinal Research Reviews·Heike J WobstJames Shorter
Sep 23, 2020·Open Biology·Benita Turner-BridgerJean-Michel Cioni
Apr 10, 2020·Frontiers in Neuroscience·Naoki SuzukiMasashi Aoki
May 16, 2020·International Journal of Molecular Sciences·Bongmin Bae, Pedro Miura
Nov 7, 2019·International Journal of Molecular Sciences·Izzy Owen, Frank Shewmaker
Dec 12, 2019·Frontiers in Molecular Biosciences·Kavya Vinayan Pushpalatha, Florence Besse
Oct 21, 2020·Emerging Topics in Life Sciences·Erik W Martin, Alex S Holehouse
Dec 9, 2020·Nature Reviews. Neuroscience·Irene Dalla CostaJeffery L Twiss
Jan 31, 2021·International Journal of Molecular Sciences·Anastasia Fomicheva, Eric D Ross
Feb 15, 2021·Biophysical Journal·Theodora Myrto PerdikariJeetain Mittal
May 4, 2020·Brain Research·Terika P SmithJeffery L Twiss
Oct 2, 2020·Physiological Reviews·Rico SchieweckMichael A Kiebler
Feb 8, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·April L Darling, James Shorter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.