Inhibition of axoplasmic transport in the rat vagus nerve alters the numbers of neuropeptide and tyrosine hydroxylase messenger RNA-containing and immunoreactive visceral afferent neurons of the nodose ganglion

Neuroscience
H ZhuoC J Helke

Abstract

Previous work showed that axotomy-induced deafferentation of the placode-derived visceral afferent neurons of the nodose ganglion altered their expression of some neuropeptides and tyrosine hydroxylase. The present studies were designed to selectively evaluate the loss of axonal transport on the numbers of vasoactive intestinal polypeptide, tyrosine hydroxylase, and calcitonin gene-related peptide mRNA-containing and immunoreactive neurons in the nodose ganglion of the adult rat. Vinblastine (0.15 mM) application to the cervical vagus nerve was used to block axonal transport between ganglionic perikarya and peripheral targets. In situ hybridization histochemistry with 35S-labeled oligonucleotide probes was used to both quantify the number of mRNA-containing neurons and to assess the density of mRNA expression per neuron, and immunocytochemistry was used to visualize the number of immunoreactive neurons. The efficacy of vinblastine to inhibit axonal transport was verified by evaluating the build-up of calcitonin gene-related peptide immunoreactive in the vagus nerve immediately rostral to the site of drug application. The absence of vinblastine-induced neuronal damage was verified by the relative absence of degenerating nerves i...Continue Reading

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Citations

Jun 1, 1997·Progress in Neurobiology·H ZhuoC J Helke
Oct 13, 2001·Trends in Microbiology·S Ohka, A Nomoto
Mar 22, 2014·Physiology & Behavior·Guillaume de Lartigue
Nov 17, 2007·The Journal of Physiology·Andrew Dilley, Geoffrey M Bove
Sep 17, 2013·The Journal of Pain : Official Journal of the American Pain Society·Andrew DilleyGeoffrey M Bove
Nov 3, 2009·The Journal of Comparative Neurology·Laurent GautronJoel Elmquist
Sep 3, 2017·Polish Journal of Veterinary Sciences·W SienkiewiczM Marszałek
Mar 16, 2013·Physiological Research·K Kaczyńska, M Szereda-Przestaszewska
Jan 12, 2001·Journal of Neurophysiology·E LancasterD Weinreich
Jan 11, 2001·Muscle & Nerve·A MegighianD Danieli-Betto
Aug 12, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ying Li, Chung Owyang
Feb 22, 2018·Journal of Neurophysiology·Ieva SatkeviciuteAndrew Dilley
Nov 24, 2020·PloS One·Michal ZaleckiAmelia Franke-Radowiecka

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