PMID: 6406265May 8, 1983Paper

Inhibition of bacterial aminopropyltransferases by S-adenosyl-1,8-diamino-3-thiooctane and by dicyclohexylamine

FEBS Letters
A E PeggJ K Coward

Abstract

Bacterial aminopropyltransferases from Escherichia coli, Serratia marcescens and Pseudomonas aeruginosa were strongly inhibited by S-adenosyl-1,8-diamino-3-thiooctane (AdoDATO) and by dicyclohexylamine. The sensitivity to these drugs in vitro was comparable to that of mammalian spermidine synthase, but AdoDATO was much less potent in reducing spermidine content in the bacteria than in mammalian cells. Although AdoDATO was a stronger inhibitor than dicyclohexylamine in vitro, dicyclohexylamine was more active in reducing bacterial spermidine levels in vivo, suggesting that it is taken up better or is more stable in the cell and is the preferable compound for in vivo studies in microorganisms. The strong inhibition of spermidine synthases by AdoDATO which is a transition state analog supports the concept that these enzymes proceed by a single displacement reaction, rather than by a ping-pong mechanism.

References

Aug 1, 1981·The Biochemical Journal·A E PeggH Hibasami
Apr 15, 1980·Archives of Biochemistry and Biophysics·K Samejima, Y Nakazawa
May 1, 1980·The Biochemical Journal·H HibasamiA E Pegg
Oct 16, 1980·Biochemical and Biophysical Research Communications·K C TangJ K Coward

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Citations

Jan 1, 1987·The International Journal of Biochemistry·S SarhanN Seiler
Sep 1, 1984·Molecular and Biochemical Parasitology·A J BitontiP P McCann
Jul 5, 1985·Biochimica Et Biophysica Acta·J L MitchellP Qasba
May 1, 1995·The International Journal of Biochemistry & Cell Biology·A E PeggJ K Coward
Jul 1, 1985·Antimicrobial Agents and Chemotherapy·L G PaulinH J Pösö
Mar 14, 2014·Journal of Agricultural and Food Chemistry·Johan P van den HeeverStephen F Pernal
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·Janina SprengerSalam Al-Karadaghi
May 19, 2009·Water Environment Research : a Research Publication of the Water Environment Federation·James E AndersonSherry A Mueller
Dec 1, 2004·EcoSal Plus·Daniel Charlier, Nicolas Glansdorff
Mar 1, 1985·Microbiological Reviews·C W Tabor, H Tabor

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