Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification

Biochimica Et Biophysica Acta
Rohan DhimanSekhar Majumdar

Abstract

Virulent tubercle bacilli inhibit apoptosis to establish a safe environment within the host cells. Here, we report that NF-kappaB dependent antiapoptotic protein bfl-1/A1 plays an important role in this process. Both virulent and avirulent mycobacteria bearing THP-1 cells expressed considerable amount of bfl-1/A1 after 4 h of infection. However, after 48 h of infection, bfl-1/A1 expression was evident only in Mycobacterium tuberculosis H37Rv but not in M. tuberculosis H37Ra infected cells. When parallel experiments were performed with Human monocyte-derived macrophages (MDMs), differential expression of bfl-1/A1 mRNA was observed in case of M. tuberculosis H37Rv and M. tuberculosis H37Ra infection. siRNA mediated inhibition of bfl-1/A1 induced apoptosis in M. tuberculosis H37Rv infected THP-1 and MDMs. Reduction in intracellular mycobacterial growth was observed in bfl-1/A1 siRNA transfected, M. tuberculosis H37Rv infected THP-1 cells. Enhancement of phagosome-lysosome fusion was observed in bfl-1/A1 siRNA treated and M. tuberculosis H37Rv infected THP-1 cells. These results clearly indicated that differential expression of bfl-1/A1 in M. tuberculosis H37Rv and M. tuberculosis H37Ra infected THP-1 cells probably account for the...Continue Reading

Citations

Jul 25, 2013·Organic & Biomolecular Chemistry·Kumaresh GhoshGiannoula Theodorakopoulos
Jun 27, 2020·Frontiers in Microbiology·Muhammad Suleman RiazAnsar Ahmed Pathan
Jun 8, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Raktim BiswasAnisur Rahman Khuda-Bukhsh
Jan 26, 2013·PloS One·Stephan DreschersThorsten W Orlikowsky
Apr 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·P DandekarC-M Lehr
Nov 30, 2011·The Journal of Cell Biology·Hiroshi AshidaChihiro Sasakawa
Nov 20, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Asmita SamadderAnisur Rahman Khuda-Bukhsh
Mar 20, 2012·The International Journal of Biochemistry & Cell Biology·Rajesh Kumar DuttaSekhar Majumdar

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis