Inhibition of binding of the AB5-type enterotoxins LT-I and cholera toxin to ganglioside GM1 by galactose-rich dietary components.

Foodborne Pathogens and Disease
P M BeckerP G van Wikselaar

Abstract

Cholera, travelers' diarrhea, or colibacillosis in pigs can possibly be prevented or attenuated by dietary provision of competitive inhibitors that react with the GM1-binding sites of the enterotoxins cholera toxin (CT), human Escherichia coli heat-labile enterotoxin of serogroup I (LTh-I), and porcine LT-I (LTp-I). The interfering efficiency of natural substances with binding of the toxins to the gangliosid receptor GM1 was tested using a specially adapted GM1-coated-microtiter-well enzyme-linked immunosorbent assay. The substances tested for their GM1 displacing capacity were galactose-containing or -related saccharides from bovine milk, skim milk powder, galactan from gum arabic, food stabilizers as well as ground fenugreek seed and soy bean constituents that contain galactomannans, the galactopolysaccharides agar and agarose, and larch wood and other plant materials that contain arabinogalactans. Skim milk powder, compared with the pure milk saccharides tested, interfered to a higher extent with LTh-I (65-66% inhibition at 5 mg test substance/mL) and CT binding (63-67% inhibition at 5 mg test substance/mL) when supplied before or simultaneously with the toxins in the GM1-enzyme-linked immunosorbent assay. Ground fenugreek s...Continue Reading

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Citations

Aug 10, 2012·The British Journal of Nutrition·Jürgen ZentekWilfried Vahjen
Sep 21, 2011·Journal of Animal Physiology and Animal Nutrition·P M BeckerP G van Wikselaar
Feb 13, 2018·PLoS Pathogens·Jakob CervinUlf Yrlid
Feb 8, 2018·ACS Infectious Diseases·Amberlyn M WandsJennifer J Kohler

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Methods Mentioned

BETA
ELISA
PCR
dot
enzyme-linked immunosorbent assay

Software Mentioned

SoftMax Pro
GenStat

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