Inhibition of binding of tomato yellow leaf curl virus rep to its replication origin by artificial zinc-finger protein.

Molecular Biotechnology
Tomoaki MoriTakashi Sera

Abstract

Previously we demonstrated that inhibition of replication-associated protein (Rep) binding to its replication origin by artificial zinc-finger proteins (AZPs) is a powerful method to prevent plant virus infection in vivo. In the present study, we applied the AZP technology to Tomato yellow leaf curl virus (TYLCV), which is a limiting factor in tomato cultivation worldwide. First, we determined 5'-ATCGGTGT ATCGGTGT-3' in the 195-bp intergenic region of the TYLCV-Israel strain, a strain reported first among TYLCV strains, as the Rep-binding site by gel shift assays. We then constructed a 6-finger AZP that bound to a 19-bp DNA including the Rep-binding site. We demonstrated that the binding affinity of the AZP was >1,000-fold greater than that of Rep and that the AZP inhibited Rep binding completely in vitro. Because the binding capability of the AZP was same as that of the AZP previously designed for geminivirus-resistant Arabidopsis thaliana, we predict that the present AZP will prevent TYLCV infection in vivo.

References

May 1, 1992·The Plant Cell·E P FontesL Hanley-Bowdoin
Mar 1, 1994·The Plant Cell·E P FontesL Hanley-Bowdoin
Nov 1, 1996·Nature Structural Biology·C A Kim, J M Berg
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S Kim, C O Pabo

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Citations

Sep 13, 2016·Frontiers in Microbiology·Ahmed HadidiMarina Barba
Feb 9, 2017·Frontiers in Microbiology·Gustavo Romay, Claude Bragard
Oct 6, 2018·Plant Signaling & Behavior·Manal TashkandiMagdy M Mahfouz
Nov 12, 2015·Genome Biology·Zahir AliMagdy M Mahfouz
Feb 6, 2015·Molecular Biology Reports·Pranav Pankaj Sahu, Manoj Prasad
Nov 24, 2016·Frontiers in Plant Science·Syed Shan-E-Ali ZaidiMagdy M Mahfouz
Apr 17, 2020·Molecular Biology Reports·Parisa Ghorbani FaalAmin Mirshamsi Kakhki
Aug 28, 2020·Frontiers in Plant Science·Kayla Beam, José Trinidad Ascencio-Ibáñez
May 31, 2019·Virusdisease·Akhilesh Kumar Kushawaha, Indranil Dasgupta
Nov 10, 2017·Emerging Topics in Life Sciences·Jorge Martínez-FortúnHuw D Jones
Aug 2, 2019·Journal of Agricultural and Food Chemistry·José S Mendoza-FigueroaManuel Soriano-García
Sep 1, 2021·Biochemistry·J Trinidad Ascencio-Ibáñez, Benjamin G Bobay

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