Inhibition of biological activity of multiplication-stimulating activity by binding to its carrier protein

Proceedings of the National Academy of Sciences of the United States of America
D J Knauer, G L Smith

Abstract

Multiplication-stimulating activity (MSA) produced by Buffalo rat liver cells (BRL-3A) in culture is related to the somatomedin family of growth regulatory polypeptides. MSA will stimulate glucose transport and DNA synthesis in normal chicken embryo fibroblasts (CEF) at concentrations of 10-200 ng/ml. MSA found in BRL-3A-conditioned medium, like the somatomedins in serum, does not exist as the free hormone but is bound to a specific high molecular weight carrier protein. In this report we demonstrate that purified MSA carrier protein (MCP) inhibits the biological activity of MSA on CEF as measured by the stimulation of glucose transport and DNA synthesis. In addition, purified MCP competitively inhibits the binding of 125I-labeled MSA to these cells. In control experiments in which insulin was used as the mitogenic agent, MCP had no effect on these biological responses. These results indicate that the inhibitory effect of MCP is the result of specific interaction with MSA and support the hypothesis that cells may be unresponsive to somatomedins bound to their serum carrier proteins.

References

Jun 1, 1975·Archives of Biochemistry and Biophysics·J ZapfE R Froesch
Jan 1, 1977·The Journal of Clinical Endocrinology and Metabolism·U KaufmannE R Froesch
May 1, 1977·The Journal of Clinical Endocrinology and Metabolism·R H ChochinovW H Daughaday
Nov 1, 1977·The Journal of Clinical Endocrinology and Metabolism·R L Hintz, F Liu
Dec 1, 1975·The Journal of Clinical Investigation·P L Poffenbarger
Jan 14, 1972·Nature·W H DaughadayJ J van Wyk

❮ Previous
Next ❯

Citations

Aug 1, 1997·Journal of Cellular Biochemistry·R H McCusker, D R Clemmons
Jan 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J PfeilschifterG R Mundy
Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G L WongS J Brandes
Nov 1, 1991·Journal of Cellular Physiology·P G Campbell, J F Novak
Jan 1, 1992·Breast Cancer Research and Treatment·J A Figueroa, D Yee
Nov 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T L RissD A Sirbasku
Sep 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R Weinstein, K Wenc
Jan 1, 1985·The International Journal of Biochemistry·H J CornellA C Herington
Feb 1, 1984·Molecular and Cellular Endocrinology·G L Smith
Dec 21, 1990·Molecular and Cellular Endocrinology·J M Luo, L J Murphy
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R C Baxter
Jan 1, 1989·Progress in Growth Factor Research·R C Baxter, J L Martin
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·L J Murphy
Nov 1, 1988·Baillière's Clinical Endocrinology and Metabolism·C T Roberts, D Leroith
Mar 1, 1997·Cytokine & Growth Factor Reviews·D R Clemmons
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E W RainesR Ross
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A BuckleyS C Woodward
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A De VroedeP G Katsoyannis
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R G ElginD R Clemmons
May 1, 1982·The Journal of Cell Biology·M J Evinger-HodgesJ R Florini
Oct 1, 1992·Molecular Biology of the Cell·R Flaumenhaft, D B Rifkin
Jan 1, 1991·Annals of the New York Academy of Sciences·L J MurphyB Bhavnani
Jan 1, 1991·Annals of the New York Academy of Sciences·L C GiudiceJ C Irwin
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S BoonenD J Baylink
Jan 1, 1989·Connective Tissue Research·T L McCarthyE Canalis
Oct 1, 1987·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D R PowellR L Hintz
Jul 1, 1983·Journal of Steroid Biochemistry·D W SchombergJ S Mondschein
Apr 15, 1991·Biochemical and Biophysical Research Communications·D L Andress, R S Birnbaum

❮ Previous
Next ❯

Related Concepts

Related Feeds

© 2021 Meta ULC. All rights reserved