Inhibition of biosynthesis and activity of nitrogenase in Azospirillum brasilense Sp7 under salinity stress

Current Microbiology
Anil Kumar TripathiDaniel Le Rudulier

Abstract

Azospirillum brasilense is a microaerophilic, plant growth-promoting bacterium, whose nitrogenase activity has been shown to be sensitive to salinity stress. Growth of A. brasilense in semi-solid medium showed that diazotrophic growth in N-free medium was relatively less sensitive to high NaCl concentrations (200-400 mM) than that in presence of NH4+. Increase in salinity stress to diazotrophic A. brasilense in the semi-solid medium led to the migration of the pellicle to deeper anaerobic zones. Assays of acetylene reduction and nifH- lacZ and nifA- lacZ fusions indicated that salinity stress inhibited nitrogenase biosynthesis more strongly than nitrogenase activity. Under salt stress, the amount of dinitrogenase reductase inactivated by ADP-ribosylation was strongly reduced, indicating that the dinitrogenase reductase ADP ribosyl transferase (DRAT) activity was also inhibited by increased NaCl concentrations. Movement of the pellicle to the anaerobic zone and inhibition of DRAT might be adaptive responses of A. brasilense to salinity stress under diazotrophic conditions. Supplementation of glycine betaine, which alleviates salt stress, partially reversed both responses.

Citations

Mar 7, 2007·Molecular Genetics and Genomics : MGG·Thirunavukkarasu NagarajanAnil Kumar Tripathi
Apr 20, 2006·Antonie van Leeuwenhoek·Inmaculada LlamasEmilia Quesada
Jan 25, 2006·Journal of Applied Microbiology·V Tsagou, G Aggelis
Nov 6, 2014·Environmental Science and Pollution Research International·Shah FahadJianliang Huang
Dec 13, 2006·FEMS Microbiology Letters·Soumitra Paul ChowdhuryAnil Kumar Tripathi
Feb 25, 2005·FEMS Microbiology Letters·Montserrat ArgandoñaAna del Moral
Jan 29, 2019·Molecular Plant-microbe Interactions : MPMI·Vijay Shankar SinghAnil Kumar Tripathi
May 15, 2012·Journal of Basic Microbiology·Diby Paul
Apr 30, 2019·Indian Journal of Microbiology·Umesh PankajRajesh Kumar Verma
Jul 8, 2018·Current Microbiology·Romina MolinaFabricio Cassán

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