Inhibition of brassinosteroid biosynthesis by either a dwarf4 mutation or a brassinosteroid biosynthesis inhibitor rescues defects in tropic responses of hypocotyls in the arabidopsis mutant nonphototropic hypocotyl 4.

Plant Physiology
Daisuke NakamotoKotaro T Yamamoto

Abstract

The nonphototropic hypocotyl 4 (nph4)/auxin response factor 7 (arf7) mutant of Arabidopsis (Arabidopsis thaliana) is insensitive to auxin and has defects in hypocotyl tropism, hook formation, differential leaf growth, and lateral root formation. To understand an auxin-signaling pathway through NPH4, we carried out screening of suppressor mutants of nph4-103 and obtained a dwarf suppressor mutant, suppressor of nph4 (snp2). snp2 had short hypocotyls in the dark condition and dark green and round leaves, short petioles, and more lateral shoots than the wild type in the light condition. The snp2 phenotypes were rescued by adding brassinolide to the growth medium in both light and dark conditions. Genetic mapping, sequence analysis, and a complementation test indicated that snp2 was a weak allele of DWARF4 (DWF4), which functions in brassinosteroid (BR) biosynthesis. snp2, which was renamed dwf4-101, exhibited photo- and gravitropisms of hypocotyls similar to those of the wild type with a slightly faster response in gravitropism. dwf4-101 almost completely suppressed defects in both tropisms of nph4-103 hypocotyls and completely suppressed hyponastic growth of nph4-103 leaves. Treatment with brassinazole, an inhibitor of BR biosynt...Continue Reading

References

May 1, 1996·Plant Physiology·S Abel, A Theologis
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G EphritikhineH Barbier-Brygoo
Feb 13, 2001·Current Opinion in Plant Biology·T Berleth, T Sachs
Jul 13, 2002·Plant Physiology·Carsten MüssigThomas Altmann
Nov 13, 2002·Plant Physiology·Hideki GodaShigeo Yoshida
Sep 25, 2003·Annual Review of Plant Biology·Shozo Fujioka, Takao Yokota
Jan 20, 2004·Plant Physiology·Joshua J BlakesleeAngus S Murphy
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Clint Chapple
Aug 26, 2004·PLoS Biology·Jennifer L NemhauserJoanne Chory
Feb 11, 2005·The Plant Journal : for Cell and Molecular Biology·Gyung-Tae KimHirokazu Tsukaya
Aug 13, 2005·The International Journal of Developmental Biology·C Alex EsmonEmmanuel Liscum
Nov 1, 1992·Plant Physiology·S D ClouseM E Baker
Jan 1, 2002·The Arabidopsis Book·Hirokazu Tsukaya

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Citations

Nov 5, 2008·Plant Molecular Biology·Hidehiro Fukaki, Masao Tasaka
May 17, 2011·Plant Physiology·Filip VandenbusscheDominique Van der Straeten
Jun 13, 2012·Plant Physiology·Aditi GuptaAshverya Laxmi
Dec 27, 2011·Journal of Plant Physiology·Rujia ZhangPedro S C Ferreira da Rocha
Oct 2, 2007·Trends in Cell Biology·Christian S HardtkeRichard Sibout
May 7, 2011·Journal of Integrative Plant Biology·Zhihong PengChunmei Ren
Sep 8, 2011·The Plant Journal : for Cell and Molecular Biology·Hinako TakehisaYoshiaki Nagamura
Apr 20, 2010·Molecular Plant·Stephen D Deslauriers, Paul B Larsen
Mar 1, 2008·Molecular Plant·Ivan A PaponovKlaus Palme
Nov 16, 2012·American Journal of Botany·Filip VandenbusscheDominique Van Der Straeten
Nov 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Eleonore HolzwartSebastian Wolf
Oct 16, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christian S Hardtke
May 14, 2017·Scientific Reports·Jun Sakaguchi, Yuichiro Watanabe

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