Inhibition of Breast Cancer Cell Migration by Cyclotides Isolated from Pombalia calceolaria.

Journal of Natural Products
Meri Emili F PintoVanderlan S Bolzani

Abstract

Two new bracelet cyclotides from roots of Pombalia calceolaria with potential anticancer activity have been characterized in this work. The cyclotides Poca A and B (1 and 2) and the previously known CyO4 (3) were de novo sequenced by MALDI-TOF/TOF mass spectrometry (MS). The MS2 spectra were examined and the amino acid sequences were determined. The purified peptides were tested for their cytotoxicity and effects on cell migration of MDA-MB-231, a triple-negative breast cancer cell line. The isolated cyclotides reduced the number of cancer cells by more than 80% at 20 μM, and the concentration-related cytotoxic responses were observed with IC50 values of 1.8, 2.7, and 9.8 μM for Poca A (1), Poca B (2), and CyO4 (3), respectively. Additionally, the inhibition of cell migration (wound-healing assay) exhibited that CyO4 (3) presents an interesting activity profile, in being able to inhibit cell migration (50%) at a subtoxic concentration (2 μM). The distribution of these cyclotides in the roots was analyzed by MALDI imaging, demonstrating that all three compounds are present in the phloem and cortical parenchyma regions.

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Citations

Jan 4, 2019·Protein and Peptide Letters·Esteban N LorenzonEduardo M Cilli
Apr 24, 2019·Biomedicines·Julio A Camarero, Maria Jose Campbell
Mar 7, 2020·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lovekesh MehtaSanjay Kumar
May 18, 2020·The Journal of Biological Chemistry·Qingdan DuDavid J Craik
Apr 30, 2021·Botanical Studies·Junpeng LiXingyong Yang
Jul 27, 2021·Journal of Natural Products·Edin MuratspahićChristian W Gruber
Jan 6, 2021·Journal of Natural Products·Meri Emili F PintoDavid J Craik
Aug 31, 2021·RSC Chemical Biology·Edin MuratspahićDavid J Craik

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Datasets Mentioned

BETA
MT576

Software Mentioned

Sigmaplot
SCiLS
Data Explorer
Systat
FleXcontrol
ImageJ
ImagePrep

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