PMID: 8606797Nov 1, 1995Paper

Inhibition of Ca2+ channel currents in human neuroblastoma (SH-SY5Y) cells by neuropeptide Y and a novel cyclic neuropeptide Y analogue

Neuropharmacology
R L McDonaldC Peers

Abstract

Whole-cell Ca2+ channel currents were recorded in human neuroblastoma (SH-SY5Y) cells, using conventional and perforated-patch techniques. Neuropeptide Y (NPY, 10-1000 nM) caused concentration-dependent inhibition of Ca2+ channel current amplitudes which was pertussis toxin-sensitive, voltage-dependent and associated with slowing of channel activation kinetics, regardless of which recording configuration was used. Inhibition was observed in all cells tested. Similar current inhibitions were observed with NPY (18-36) and peptide YY, but not with [Leu31, Pro34]NPY, indicating that the effects were mediated by Y2 receptors. Pancreatic polypeptide (100 nM) was without effect on Ca2+ channel currents. The effects of NPY were additive with nifedipine (at a supramaximal concentration of 5 microM), suggesting that NPY predominantly inhibits N-type Ca2+ channels present in these cells, and not L-type. The effects of NPY were mimicked by a novel, cyclic analogue of NPY which is 40-fold more selective for Y2 receptors than other commonly used Y2-selective peptides. The cyclic analogue was also more potent than NPY itself, causing maximal current inhibition at approx 300 nM, whereas the response to NPY was not fully saturated at 1 microM. ...Continue Reading

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Citations

Mar 5, 1999·Molecular Neurobiology·P F VaughanC Peers
Nov 5, 1998·Regulatory Peptides·L A McCulloughT C Westfall
Aug 5, 1999·British Journal of Pharmacology·A P Serone, J A Angus
Nov 13, 2007·Journal of Molecular and Cellular Cardiology·Neil HerringDavid J Paterson
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Martin M HöfligerAnnette G Beck Sickinger
Sep 4, 2014·The Journal of Biological Chemistry·Kévin CarayonCatherine Mollereau
Apr 1, 2020·Cell Communication and Signaling : CCS·Isabelle ZiffertAnnette G Beck-Sickinger
Jun 5, 1998·The American Journal of Physiology·L A McCulloughT C Westfall
Jan 17, 1997·Journal of Medicinal Chemistry·D A KirbyJ E Rivier

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