PMID: 7527459Aug 15, 1994Paper

Inhibition of Ca2+ entry by Ca2+ overloading of intracellular Ca2+ stores in human platelets

The Journal of Physiology
M KimuraA Aviv

Abstract

1. This study examined the effect of overloading human platelet intracellular Ca2+ stores on the rate of agonist-evoked external Ca2+ entry. To overload the Ca2+ stores (presumably dense tubules), external Na(+)-dependent Ca2+ efflux via Na(+)-Ca2+ exchange was inhibited by pretreating the cells with ouabain or Na(+)-free medium. Ca2+ regulation was then examined after exposure to thrombin, ADP and thapsigargin. Cytosolic free Ca2+ levels were monitored using the fluorescent probe fura-2 and external Ca2+ influx was assessed by the rates of extracellular Mn2+ or 45Ca2+ uptake. 2. Both ouabain and Na(+)-free pretreatments caused a slight increase in the resting cytosolic free Ca2+. 3. In 1 mM Ca(2+)-containing medium, Ca(2+)-overloaded platelets showed similar thrombin-evoked cytosolic free Ca2+ responses to those of control platelets. However, in Ca(2+)-free medium, they showed substantially greater thrombin-evoked cytosolic free Ca2+ responses than control platelets. Moreover, increased thrombin-evoked Ca2+ mobilization from Ca2+ storage sites was accompanied by a diminished rate of thrombin-evoked external Ca2+ entry. 4. Similar reductions in the rate of external Ca2+ entry were observed after treatment with ADP and thapsigar...Continue Reading

Citations

Oct 3, 1999·Progress in Cardiovascular Diseases·L Resnick
Aug 14, 2013·PloS One·Andrew V BenestHellmut G Augustin
Jun 3, 2005·American Journal of Physiology. Cell Physiology·Masayuki KimuraAbraham Aviv
Jan 13, 2000·Biochemical and Biophysical Research Communications·O A Gende

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