PMID: 8584431Jan 1, 1996Paper

Inhibition of calmodulin expression prevents low-pH-induced gap junction uncoupling in Xenopus oocytes

Pflügers Archiv : European journal of physiology
C PeracchiaL L Peracchia

Abstract

The relationship among intracellular pH (pHi), -log10 intracellular Ca2+ concentration (pCai) and gap junctional conductance, the participation of Ca2+ stores, and the role of calmodulin in channel regulation have been studied in Xenopus oocytes, expressing the native connexin (Cx38), exposed to external solutions bubbled with 100% CO2. The time courses of pHi [measured with 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorscein (BCECF)], pCai (measured with the membrane-associated fura-C18) and junctional conductance (measured with a double voltage-clamp protocol) were compared. The data obtained confirm previous evidence for a closer relationship of junctional conductance with pCai than with pHi. Evidence for an inhibitory effect of intracellularly injected ruthenium red or 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) on CO2-induced uncoupling, coupled to negative results with Ca2+-free external solutions, point to a low-pHi -induced Ca2+ release from internal stores, likely to be primarily mitochondria. The hypothesis proposing a participation of calmodulin in channel gating was tested by studying the effects of calmodulin expression inhibition by intracellular injection of oligonucleotides antisense to the two ...Continue Reading

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Citations

Jun 30, 2005·The Journal of Membrane Biology·B A Kotsias, C Peracchia
Jun 15, 2007·The Journal of Membrane Biology·Camillo PeracchiaLillian L Peracchia
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Apr 1, 1996·Pflügers Archiv : European journal of physiology·X WangC Peracchia
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Oct 1, 2017·The Biochemical Journal·Juan ZouJenny J Yang
Jul 17, 2020·International Journal of Molecular Sciences·Camillo Peracchia
Jan 17, 2020·International Journal of Molecular Sciences·Camillo Peracchia
Jun 11, 1999·The American Journal of Physiology·C PeracchiaL L Peracchia
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Nov 1, 1996·The American Journal of Physiology·X G Wang, C Peracchia
Mar 23, 2004·Biochimica Et Biophysica Acta·Camillo Peracchia

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