Inhibition of Candida albicans morphogenesis by chitinase from Lactobacillus rhamnosus GG

Scientific Reports
Camille N AllonsiusSarah Lebeer

Abstract

Lactobacilli have been evaluated as probiotics against Candida infections in several clinical trials, but with variable results. Predicting and understanding the clinical efficacy of Lactobacillus strains is hampered by an overall lack of insights into their modes of action. In this study, we aimed to unravel molecular mechanisms underlying the inhibitory effects of lactobacilli on hyphal morphogenesis, which is a crucial step in C. albicans virulence. Based on a screening of different Lactobacillus strains, we found that the closely related taxa L. rhamnosus, L. casei and L. paracasei showed stronger activity against Candida hyphae formation compared to other Lactobacillus species tested. By exploring the activity of purified compounds and mutants of the model strain L. rhamnosus GG, the major peptidoglycan hydrolase Msp1, conserved in the three closely related taxa, was identified as a key effector molecule. We could show that this activity of Msp1 was due to its ability to break down chitin, the main polymer in the hyphal cell wall of C. albicans. This identification of a Lactobacillus-specific protein with chitinase activity having anti-hyphal activity will assist in better strain selection and improved application in futur...Continue Reading

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Citations

Sep 8, 2019·World Journal of Microbiology & Biotechnology·Bao Le, Seung Hwan Yang
Aug 8, 2020·Frontiers in Microbiology·Marie LegeinSarah Lebeer
May 16, 2020·Scientific Reports·Eline F M OerlemansSarah Lebeer
Jun 20, 2020·Microbial Cell Factories·Priscilla Romina De GregorioBeatrice Vitali
Nov 9, 2020·Microbial Cell Factories·Wallace Jeng Yang CheeLeslie Thian Lung Than
Oct 19, 2019·Microbial Pathogenesis·Lingzhi NiuYajuan Zheng
Mar 12, 2021·Biochemical Society Transactions·Lize DelangheSarah Lebeer
Mar 9, 2021·Computational and Structural Biotechnology Journal·Raghav VijSascha Brunke
Aug 28, 2021·Trends in Microbiology·Ronit SuissaIlana Kolodkin-Gal

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Methods Mentioned

BETA
light microscopy
ELISA
glycosylation
size exclusion chromatography
protein assay

Software Mentioned

AxioVision
BLAST
GraphPad Prism

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