Inhibition of CD147 expression by RNA interference reduces proliferation, invasion and increases chemosensitivity in cancer stem cell-like HT-29 cells

International Journal of Oncology
Jie ChenShukui Wang

Abstract

The association between CD147 and cancer stem cells (CSCs) provides a new angle for cancer treatments. The aim of this study was to investigate the biological roles of CD147 in colorectal CSCs. The Oct4-green fluorescent protein (GFP) vector was used to isolate CSCs and pYr-mir30-shRNA was used to generate short hairpin RNA (shRNA) specifically for CD147. After RNA interference (RNAi), CD147 was evaluated by reverse transcription‑quantitative PCR and western blot analysis, and its biological functions were assessed by MTT and invasion assays. The results showed that the differentiation of isolated CSC-like HT-29 cells was blocked and these cells were highly positive for CD44 and CD147. RNAi-mediated CD147 silencing reduced the expression of CD147 at both mRNA and protein levels. Moreover, the activities of proliferation and invasion were decreased obviously in CSCs. Knockdown of CD147 increased the chemosensitivity of CSC-like cells to gemcitabine, cisplatin, docetaxel at 0.1, 1 and 10 µM respectively, however, there was no significant difference among the three groups to paclitaxel at 10 µM. In conclusion, these results suggest that CD147 plays an important role in colorectal CSCs and might be regarded as a novel CSC-specific ...Continue Reading

References

Mar 29, 1990·Nature·H R SchölerP Gruss
Dec 15, 1982·Biochemical and Biophysical Research Communications·C Biswas
May 10, 2003·The Journal of Biological Chemistry·Suniti MisraBryan P Toole
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Feb 2, 2006·Stem Cells·Grant A Challen, Melissa H Little
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Dec 10, 2008·Blood·John E Dick
Mar 3, 2009·Seminars in Radiation Oncology·Maximilian DiehnMichael F Clarke
May 28, 2010·Stem Cells·Gangadharan B SajithlalEdward V Prochownik
Jun 12, 2010·PloS One·Dwain K IrvinChristopher J Wheeler
Jul 28, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sarina R CameronNicholas A Saunders
Dec 31, 2010·Research in Veterinary Science·Masaki MichishitaKimimasa Takahashi
Mar 10, 2011·Nature Medicine·Hans Clevers
May 31, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marcello Maugeri-SaccàRuggero De Maria
Jun 28, 2011·Gastroenterology·Kuo-Shu TsaiShih-Chieh Hung
Jun 28, 2011·The EMBO Journal·Ying-Jhen SuJia-Lin Lee
Nov 26, 2011·Cell Death and Differentiation·M BartucciR De Maria
May 23, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Suzanne GeorgeGeorge D Demetri
Feb 28, 2013·International Journal of Molecular Sciences·Wenjing JiangKunlin Jin
Mar 14, 2013·Oncoimmunology·Erika VacchelliLorenzo Galluzzi
Apr 4, 2013·Nature Cell Biology·Jan Paul Medema
Sep 3, 2013·Expert Review of Proteomics·Kristine M Kim, Eugene C Yi
Dec 10, 2013·Diseases of the Colon and Rectum·M WassermanA L Halverson
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 28, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Kiyoshi HigashiKoichi Saito

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