Inhibition of CD26/DPP-IV enhances donor muscle cell engraftment and stimulates sustained donor cell proliferation.

Skeletal Muscle
Maura H ParkerStephen J Tapscott

Abstract

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx5cv mice. Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftme...Continue Reading

References

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Citations

Jun 19, 2014·Skeletal Muscle·Rabi TawilStephen J Tapscott
Feb 5, 2013·The FEBS Journal·Dane K Lund, D D W Cornelison
May 11, 2013·Current Protocols in Stem Cell Biology·Maura H Parker, Stephen J Tapscott
Apr 19, 2013·Current Opinion in Hematology·Heather O'LearyHal E Broxmeyer
Mar 12, 2021·Veterinary Pathology·Joanne L TuohyBernard Seguin

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