Inhibition of cell cycle progression and migration of vascular smooth muscle cells by prostaglandin D2 synthase: resistance in diabetic Goto-Kakizaki rats

American Journal of Physiology. Cell Physiology
Louis RagoliaJohn K Maesaka

Abstract

The regulation of vascular smooth muscle cell (VSMC) proliferation, migration, and apoptosis plays a clear role in the atherosclerotic process. Recently, we reported on the inhibition of the exaggerated growth phenotype of VSMCs isolated from hypertensive rats by lipocalin-type prostaglandin D2 synthase (L-PGDS). In the present study, we report the differential effects of L-PGDS on VSMC cell cycle progression, migration, and apoptosis in wild-type VSMCs vs. those from a type 2 diabetic model. In wild-type VSMCs, exogenously added L-PGDS delayed serum-induced cell cycle progression from the G1 to S phase, as determined by gene array analysis and the decreased protein expressions of cyclin-dependent kinase-2, p21(Cip1), and cyclin D1. Cyclin D3 protein expression was unaffected by L-PGDS, although its gene expression was stimulated by L-PGDS in wild-type cells. In addition, platelet-derived growth factor-induced VSMC migration was inhibited by L-PGDS in wild-type cells. Type 2 diabetic VSMCs, however, were resistant to the L-PGDS effects on cell cycle progression and migration. L-PGDS did suppress the hyperproliferation of diabetic cells, albeit through a different mechanism, presumably involving the 2.5-fold increase in apoptosi...Continue Reading

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Citations

Dec 5, 2008·American Journal of Physiology. Cell Physiology·Jin Hee LeeLouis Ragolia
Nov 19, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yoshikazu MiwaToshiyuki Sasaguri
Feb 11, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Louis RagoliaArzu Büyük
Jul 16, 2008·Prostaglandins & Other Lipid Mediators·Louis RagoliaThomas Palaia
May 30, 2006·Animal Reproduction Science·De-Yu ChenYu-Feng Huang
Dec 18, 2014·International Journal of Cancer. Journal International Du Cancer·Tatsunari FukuokaKosei Hirakawa
May 3, 2013·Clinical Science·Karen E Porter, Kirsten Riches
Jun 23, 2005·The Journal of Biological Chemistry·Louis RagoliaYoshihiro Urade
Nov 12, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Sukrutha ChettimadaSachin A Gupte
Sep 10, 2019·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Dongdong ChaiHong Jiang

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