Inhibition of cell proliferation and activation of protein tyrosine phosphatase mediated by angiotensin II type 2 (AT2) receptor in R3T3 cells

Biochemical and Biophysical Research Communications
S TsuzukiT Inagami

Abstract

Angiotensin II (Ang II) type 2 receptor (AT2) mediated inhibition of cell proliferation and activation of protein tyrosine phosphatase (PTP) were investigated in R3T3 fibroblast cells selectively expressing the AT2 subtype. Ang II did not alter the cell number of serum depleted R3T3 cells but inhibited basic fibroblast growth factor (bFGF)-stimulated cell proliferation in a dose dependent manner (IC50; 1-5 nM). This inhibitory response was abolished by an concomitant incubation with an AT2 antagonist, PD123319, and was mimicked by an AT2 agonist, CGP42112A. Stimulation of Ang II resulted in a rapid and transient increase in PTP activity as determined by using para-nitrophenyl phosphate (p-Npp) as a substrate in serum depleted R3T3 cells. This PTP activation was mimicked by CGP42112A. We conclude that the AT2 receptor is involved in the inhibition of cell proliferation and activation of protein tyrosine phosphatase.

Citations

Sep 3, 2010·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Li YuLie Gao
Apr 2, 2002·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masahisa GotoKazuwa Nakao
Jan 12, 1999·British Journal of Pharmacology·A S ZwartR E Widdop
Oct 8, 2003·British Journal of Pharmacology·Robert E WiddopTracey A Gaspari
Sep 1, 2004·Brain Research. Molecular Brain Research·William R SchelmanJames A Weyhenmeyer
Apr 20, 2004·Brain Research. Molecular Brain Research·Steven A MooreJames A Weyhenmeyer
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B PetersJ Peters
Dec 5, 2002·The Journal of Biological Chemistry·Louis GendronNicole Gallo-Payet
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·C M FilipeanuD de Zeeuw
Oct 3, 1999·Cell Biochemistry and Biophysics·D H Wang, F Elijovich
Dec 22, 1999·The Journal of Clinical Endocrinology and Metabolism·E ChamouxN Gallo-Payet
Jan 15, 2004·The Journal of Pharmacology and Experimental Therapeutics·Bradley T AndresenEdwin K Jackson
Feb 24, 1999·Journal of Cardiovascular Pharmacology·U Stroth, T Unger
Feb 27, 2001·Current Opinion in Nephrology and Hypertension·A BlumeT Unger
Jul 5, 2018·International Journal of Endocrinology·Alexandre ConnollyJean-Patrice Baillargeon
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·C BerryD G Johns
Feb 10, 1999·The American Journal of Physiology·P E McEwanC J Kenyon
Mar 7, 2000·Journal of Hypertension·T Unger
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·S GallinatC Sumners
Jan 4, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H Nishimura
Jun 1, 2001·Regulatory Peptides·M Stoll, T Unger
Nov 5, 1999·Trends in Endocrinology and Metabolism : TEM·M HoriuchiL Daviet

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