Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells

FEMS Immunology and Medical Microbiology
Tetsuya MizutaniShigeru Morikawa

Abstract

Severe acute respiratory syndrome (SARS) is caused by SARS-coronavirus (SARS-CoV). Infection of Vero E6 cells with SARS-CoV inhibits cell proliferation. Our previous study indicated that Akt, which is poorly phosphorylated in confluent cultures of Vero E6 cells, is phosphorylated and then dephosphorylated upon infection by SARS-CoV. In the present study, we showed that a serine residue of Akt was phosphorylated in Vero E6 cells in subconfluent culture and that Akt was dephosphorylated rapidly after SARS-CoV infection without up-regulation of its phosphorylation. Phosphorylation of glycogen synthase kinase-3beta, which is one of the downstream targets of Akt, was prevented in SARS-CoV-infected cells. However, treatment with glycogen synthase kinase-3beta small interfering RNA indicated that the glycogen synthase kinase-3beta signaling pathway was not related to inhibition of cell proliferation. Treatment of Vero E6 cells with the phosphatidylinositol 3'-kinase/Akt inhibitor, LY294002, which induces dephosphorylation of Akt, inhibited cell proliferation. As shown in our previous studies, apoptosis occurred in virus-infected cells within 18 h postinfection. Cellular mRNA transcription, which was reported to be up-regulated in SARS...Continue Reading

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Citations

Sep 7, 2011·Advanced Drug Delivery Reviews·Christian PlankOlga Mykhaylyk
May 6, 2020·British Journal of Pharmacology·Hong ZhouXiao-Ming Meng
Jun 12, 2020·Journal of Translational Medicine·Francesco MessinaUNKNOWN COVID 19 INMI Network Medicine for IDs Study Group
May 29, 2020·Cellular and Molecular Life Sciences : CMLS·Raghuram KogantiDeepak Shukla
Mar 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhen-Zhen WangXiu-Min Li
Aug 25, 2020·Biochemical and Biophysical Research Communications·H Y Lim Tung, Pierre Limtung
Jun 4, 2021·Journal of Medicinal Chemistry·Thanigaimalai Pillaiyar, Stefan Laufer

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Methods Mentioned

BETA
immunoprecipitation
transfection
PCR
reverse transcription PCR

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis