Jul 13, 2004

Inhibition of cell-wall autolysis and pectin degradation by cations

Plant Physiology and Biochemistry : PPB
J Bernhard WehrF Pax C Blamey

Abstract

Modification of cell wall components such as cellulose, hemicellulose and pectin plays an important role in cell expansion. Cell expansion is known to be diminished by cations but it is unknown if this results from cations reacting with pectin or other cell wall components. Autolysis of cell wall material purified from bean root (Phaseolus vulgaris L.) occurred optimally at pH 5.0 and released mainly neutral sugars but very little uronic acid. Autolytic release of neutral sugars and uronic acid was decreased when cell wall material was loaded with Ca, Cu, Sr, Zn, Al or La cations. Results were also extended to a metal-pectate model system, which behaved similarly to cell walls and these cations also inhibited the enzymatic degradation by added polygalacturonase (EC 3.2.1.15). The extent of sugar release from cation-loaded cell wall material and pectate gels was related to the degree of cation saturation of the substrate, but not to the type of cation. The binding strength of the cations was assessed by their influence on the buffer capacity of the cell wall and pectate. The strongly bound cations (Cu, Al or La) resulted in higher cation saturation of the substrate and decreased enzymatic degradability than the weakly held catio...Continue Reading

  • References8
  • Citations26

Mentioned in this Paper

Metabolic Process, Cellular
Galacturonic acid
Carbohydrate nutrients
Pellet Formation
Enzymes, antithrombotic
Pectins
Pectate
Phaseolus acutifolius
Phaseolus vulgaris
Solanum tuberosum

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