Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication

Virology
W A BresnahanT Albrecht

Abstract

Human cytomegalovirus is a herpesvirus that induces numerous cellular processes upon infection. Among these are activation of cyclin-dependent kinase 2, which regulates cell cycle progression in G1 and S phase. We report here that inhibition of cellular Cdk2 activity blocks HCMV replication. Inhibition of Cdk2 activity by roscovitine inhibits HCMV DNA synthesis, production of infectious progeny, and late antigen expression in infected cells in a dose-dependent manner. HCMV replication is also inhibited by the expression of a Cdk2 dominant negative mutant, whereas expression of wild-type Cdk2 has no effect on viral replication. These data indicate that activation of cellular Cdk2 is necessary for HCMV replication.

References

Oct 1, 1977·Journal of Virology·J E Estes, E S Huang
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R W Honess, B Roizman
Sep 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M M MonickG W Hunninghake
Sep 1, 1990·Reviews of Infectious Diseases·C A AlfordW J Britt
Sep 1, 1990·Reviews of Infectious Diseases·R H Rubin
Sep 1, 1990·Reviews of Infectious Diseases·R T Schooley
May 1, 1980·American Journal of Clinical Pathology·T Albrecht, T H Weller
Aug 1, 1995·The Journal of Cell Biology·P K JacksonM W Kirschner
Sep 1, 1994·European Journal of Biochemistry·J VeselýS Leclerc
Dec 24, 1993·Science·S van den Heuvel, E Harlow
Aug 23, 1993·Biochimica Et Biophysica Acta·P J FarnhamR Kollmar
Apr 1, 1996·Journal of Virology·P Dal MonteS Michelson
Feb 1, 1997·Journal of Virology·D Dittmer, E S Mocarski
Oct 1, 1996·Trends in Cell Biology·L Meijer

❮ Previous
Next ❯

Citations

Jun 26, 2001·International Journal of Experimental Pathology·C Swanton, N Jones
Sep 1, 2007·Current Opinion in Oncology·Dirk P Dittmer, Susan E Krown
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Sunwen ChouGail I Marousek
Nov 17, 2006·Journal of Virology·Yoshihiro IzumiyaHsing-Jien Kung
Mar 3, 2004·Journal of Virology·Shannon L TaylorJennifer F Moffat
Jan 17, 2009·Cell Division·Adam J Hume, Robert F Kalejta
Sep 17, 2010·PLoS Pathogens·Martin ZydekLüder Wiebusch
Dec 10, 2013·Antiviral Research·Yi Ling ChiaSheng-Hao Chao
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoon-Jae Song, Mark F Stinski
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Miguel Gaspar, Thomas Shenk
Apr 24, 2013·Trends in Microbiology·Renfeng Li, S Diane Hayward
Aug 31, 2010·Cellular and Molecular Life Sciences : CMLS·Martin MichaelisJindrich Cinatl
Sep 19, 2002·Trends in Pharmacological Sciences·Marie KnockaertLaurent Meijer
Sep 24, 2004·Reviews in Medical Virology·Tatsuya TsurumiAyumi Kudoh
Feb 3, 2005·Environmental and Molecular Mutagenesis·Michael SchmiedererThomas Albrecht

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.