Inhibition of cellulose enzymatic hydrolysis by laccase-derived compounds from phenols

Biotechnology Progress
Alfredo Oliva-TaravillaMercedes Ballesteros

Abstract

The presence of inhibitors compounds after pretreatment of lignocellulosic materials affects the saccharification and fermentation steps in bioethanol production processes. Even though, external addition of laccases selectively removes the phenolic compounds from lignocellulosic prehydrolysates, when it is coupled to saccharification step, lower hydrolysis yields are attained. Vanillin, syringaldehyde and ferulic acid are phenolic compounds commonly found in wheat-straw prehydrolysate after steam-explosion pretreatment. These three phenolic compounds were used in this study to elucidate the inhibitory mechanisms of laccase-derived compounds after laccase treatment. Reaction products derived from laccase oxidation of vanillin and syringaldehyde showed to be the strongest inhibitors. The presence of these products causes a decrement on enzymatic hydrolysis yield of a model cellulosic substrate (Sigmacell) of 46.6 and 32.6%, respectively at 24 h. Moreover, a decrease in more than 50% of cellulase and β-glucosidase activities was observed in presence of laccase and vanillin. This effect was attributed to coupling reactions between phenoxyl radicals and enzymes. On the other hand, when the hydrolysis of Sigmacell was performed in pr...Continue Reading

References

Jan 22, 2008·Journal of Agricultural and Food Chemistry·Loredana CanforaLiliana Gianfreda
Aug 18, 2009·Bioresource Technology·Miguel JuradoMaría Jesús Martínez
Nov 25, 2011·Enzyme and Microbial Technology·Eduardo XimenesMichael Ladisch
Nov 25, 2011·Enzyme and Microbial Technology·Tukayi KudangaStephanie Burton
Nov 25, 2011·Enzyme and Microbial Technology·Ani Tejirian, Feng Xu
Nov 25, 2011·Enzyme and Microbial Technology·Youngmi KimMichael R Ladisch
Dec 7, 2011·Enzyme and Microbial Technology·Ulla MoilanenLiisa Viikari
Nov 29, 2012·Bioresource Technology·Youngmi KimMichael R Ladisch
Sep 3, 2013·Applied Biochemistry and Biotechnology·Xuejiao SunPing Wang
Nov 28, 2013·Biotechnology for Biofuels·Mai Ostergaard Haven, Henning Jørgensen
May 21, 2014·Applied Microbiology and Biotechnology·Tukayi Kudanga, Marilize Le Roes-Hill
Dec 3, 2014·Bioresource Technology·Alfredo Oliva-TaravillaMercedes Ballesteros

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Citations

Dec 20, 2015·Journal of Biotechnology·Alfredo Oliva-TaravillaMercedes Ballesteros
Jun 6, 2016·Bioresource Technology·Daehwan KimMichael R Ladisch
Apr 11, 2018·Critical Reviews in Biotechnology·Hao LiuPratyoosh Shukla
Jul 27, 2018·Cellular and Molecular Life Sciences : CMLS·Zheng-Bing GuanXiang-Ru Liao
Jun 9, 2020·World Journal of Microbiology & Biotechnology·David J Timson
Mar 15, 2019·Biotechnology for Biofuels·Simona GiacobbeCinzia Pezzella
Mar 14, 2021·Environmental Science and Pollution Research International·Manisha Malhotra, Sunil Kumar Suman

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