PMID: 8591796Nov 1, 1995Paper

Inhibition of ciliated cell differentiation by fluid submersion

Experimental Lung Research
L E Ostrowski, P Nettesheim

Abstract

Rat tracheal epithelial (RTE) cells, plated at low density on collagen gel-coated membranes, differentiate into a mucociliary epithelium when cultured at an air-liquid interface (ALI). However, when RTE cells are cultured submerged in media, ciliated cell differentiation is drastically reduced. This study examined possible mechanisms for the inhibition of ciliated cell differentiation by submersion. Ciliated cell differentiation was measured using a monoclonal antibody specific for rat ciliated cells. Removing growth stimulatory compounds from both the basal and apical media increased ciliated cell differentiation in submerged cultures, indicating that submersion inhibits, but does not prevent, ciliogenesis. However, the effect of submersion was independent of the composition of the apical media. The depth of apical fluid was important, with depths > or = 1 mm causing almost complete inhibition of ciliated cell differentiation, while a depth of 0.5 mm allowed significant ciliogenesis. Submersion appeared to block ciliated cell differentiation at an early step, because ciliated cell development required several days following creation of an ALI. Once ciliogenesis was initiated in ALI cultures, submersion did not reverse or inhib...Continue Reading

References

Jun 1, 1992·Journal of Cellular Physiology·D MooneyD Ingber
May 1, 1992·Experimental Lung Research·T ShimizuS H Randell
Aug 1, 1992·Pediatric Pulmonology·K DeBoeckL Corbeel
Apr 1, 1992·Journal of Toxicology and Environmental Health·F ColizzoA T Hastie
Aug 1, 1991·The American Journal of Physiology·M KondoJ H Widdicombe
Jan 1, 1990·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·M JorissenJ J Cassiman
Feb 1, 1989·Current Opinion in Cell Biology·C M Wischik
Jan 1, 1989·Archives of Oto-rhino-laryngology·M JorissenJ J Cassiman
Aug 1, 1989·The American Journal of Anatomy·D A GaillardE S Puchelle
Nov 1, 1986·Infection and Immunity·S T HingleyM L Higgins
May 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M J WhitcuttR Wu
Jan 1, 1982·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K P KeenanE M McDowell
Jan 1, 1984·Experimental Lung Research·T C LeeP Nettesheim
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·A Baeza-SquibanF Marano
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L KaartinenS H Randell
Mar 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A B ClarkL E Ostrowski
Sep 1, 1993·American Journal of Respiratory Cell and Molecular Biology·J L CarsonR B Delvin
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L KaartinenS H Randell

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Citations

Jul 13, 2002·Journal of Cellular Physiology·Kenneth E ChapmanWilliam M Miller
May 7, 2008·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Eberhard StennertAxel Mickenhagen
Dec 3, 2009·Tissue Engineering. Part C, Methods·Yuchi WangHua Mao
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·L E OstrowskiP Nettesheim
Apr 7, 2007·American Journal of Respiratory Cell and Molecular Biology·Andrea J RossRobert B Devlin
May 14, 2008·American Journal of Respiratory Cell and Molecular Biology·Jennifer S GuimbellotJeong S Hong
Oct 19, 2013·The Journal of Clinical Investigation·Mahboobe GhaediLaura E Niklason
Nov 14, 2003·In Vitro Cellular & Developmental Biology. Animal·J S PaquetteF Goulet
Apr 24, 2014·American Journal of Respiratory Cell and Molecular Biology·Benjamin J GerovacNevis L Fregien
Mar 1, 1997·In Vitro Cellular & Developmental Biology. Animal·E Antoshina, L E Ostrowski
May 25, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Maria Teresa ConconiGastone Giovanni Nussdorfer
Apr 3, 2014·IEEE Transactions on Bio-medical Engineering·Elizabeth A CalleLaura E Niklason
Aug 25, 2016·Nature Communications·Matteo VillaAndreas Wack
Dec 31, 1997·The American Journal of Physiology·P D PotdarL E Ostrowski
Dec 21, 2000·American Journal of Physiology. Cell Physiology·Z BebökC J Venglarik

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