Inhibition of connexin 43 hemichannel-mediated ATP release attenuates early inflammation during the foreign body response

Tissue Engineering. Part a
Bennett W CalderMichael J Yost

Abstract

In the last 50 years, the use of medical implants has increased dramatically. Failure of implanted devices and biomaterials is a significant source of morbidity and increasing healthcare expenditures. An important cause of implant failure is the host inflammatory response. Recent evidence implicates extracellular ATP as an important inflammatory signaling molecule. A major pathway for release of cytoplasmic ATP into the extracellular space is through connexin hemichannels, which are the unpaired constituents of gap junction intercellular channels. Blockade of hemichannels of the connexin 43 (Cx43) isoform has been shown to reduce inflammation and improve healing. We have developed a Cx43 mimetic peptide (JM2) that targets the microtubule-binding domain of Cx43. The following report investigates the role of the Cx43 microtubule-binding domain in extracellular ATP release by Cx43 hemichannels and how this impacts early inflammatory events of the foreign body reaction. In vitro Cx43 hemichannel-mediated ATP release by cultured human microvascular endothelial cells subjected to hypocalcemic and normocalcemic conditions was measured after application of JM2 and the known hemichannel blocker, flufenamic acid. A submuscular silicone i...Continue Reading

References

Jul 26, 1972·Nature: New Biology·R FlowerJ R Vane
Nov 1, 1993·The Journal of Clinical Investigation·K SutherlandJ W Eaton
Oct 1, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·P Bodin, G Burnstock
Feb 27, 1999·Biochemical Society Transactions·V A FadokP M Henson
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·P A WenderJ B Rothbard
Jan 9, 2001·Journal of Cellular Physiology·R S LabowJ P Santerre
Sep 13, 2001·Current Biology : CB·B N GiepmansW H Moolenaar
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Mar 14, 2002·The Journal of Membrane Biology·S EskandariD D F Loo
Jul 20, 2002·Molecular Membrane Biology·W Howard Evans, Patricia E M Martin
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Undine LaufMatthias M Falk
Nov 16, 2002·The Journal of Physiology·Annick Guyot, John W Hanrahan
May 20, 2003·Immunological Reviews·Hans-Uwe Simon
Jul 5, 2003·Cell Biology International·Petula CoutinhoDavid Becker
Oct 3, 2003·Current Biology : CB·Cindy QiuDavid L Becker
Nov 7, 2003·American Journal of Physiology. Cell Physiology·Xiaoyong BaoLuis Reuss
Jul 17, 2004·Annual Review of Biomedical Engineering·Buddy D Ratner, Stephanie J Bryant
Aug 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott J CruikshankMiduturu Srinivas
Mar 2, 2005·Tissue Engineering·Cameron J WilsonMark J Pearcy
Mar 26, 2005·Investigative Ophthalmology & Visual Science·Priya GomesBernard Himpens
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oliver SoehnleinLennart Lindbom
Sep 15, 2005·Journal of Cell Science·Tamsin ThomasDale W Laird
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Kyathanahalli S JanardhanBaljit Singh
Dec 22, 2005·Nature Immunology·Charles N Serhan, John Savill
Feb 24, 2006·The Biochemical Journal·Dale W Laird
Dec 16, 2006·Science·Yu ChenWolfgang G Junger
Jun 27, 2007·The American Journal of Pathology·Lars-Peter Erwig, Peter M Henson
Dec 29, 2007·Seminars in Immunology·James M AndersonDavid T Chang
Feb 26, 2008·Trends in Biotechnology·J Matthew RhettRobert G Gourdie
May 21, 2008·Blood·Oliver SoehnleinLennart Lindbom
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Jan 15, 2009·Journal of Leukocyte Biology·Susan MacLauchlanThemis R Kyriakides
Mar 25, 2009·The Biochemical Journal·Joell L Solan, Paul D Lampe
Apr 22, 2009·The Journal of Clinical Investigation·Matthew C HymanDavid J Pinsky
May 2, 2009·Plastic and Reconstructive Surgery·Brent L SoderRobert G Gourdie
Jul 29, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christelle LecutCécile Oury
Mar 12, 2010·Cell Communication & Adhesion·Rodolfo IglesiasEliana Scemes
Jul 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raf PonsaertsGeert Bultynck
Aug 5, 2010·Molecular and Cellular Neurosciences·Nicolas FrogerChristian Giaume
Sep 11, 2010·International Immunopharmacology·V Kumar, A Sharma

❮ Previous
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Citations

Jul 28, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Noah A Levit, Thomas W White
Jul 9, 2016·Critical Reviews in Biochemistry and Molecular Biology·Joost WillebrordsMathieu Vinken
Nov 20, 2016·The Journal of Investigative Dermatology·Christina L GrekGautam S Ghatnekar
Jan 27, 2017·BMC Cell Biology·Daniela BegandtXavier F Figueroa
Mar 16, 2018·International Journal of Molecular Sciences·Robert D Johnson, Patrizia Camelliti
Mar 31, 2018·International Journal of Molecular Sciences·J Matthew Rhett, Elizabeth S Yeh
Sep 22, 2017·Pharmacological Reviews·Luc LeybaertRainer Schulz
Jun 22, 2017·Neurochemical Research·Marijke De BockChristian Giaume
Jul 6, 2020·Clinical Oral Investigations·Wen Yi LimDavid Laurence Becker
Jul 14, 2017·American Journal of Physiology. Cell Physiology·J Matthew RhettMichael J Yost
Jan 31, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Elisabeth ObertBärbel Rohrer
Aug 16, 2017·Scientific Reports·Joost WillebrordsMathieu Vinken
Jun 17, 2020·Expert Opinion on Drug Discovery·Anne CaufriezMathieu Vinken
Aug 28, 2020·Biomolecules·Olga M RusieckaBrenda R Kwak
Apr 20, 2018·International Journal of Molecular Sciences·Michel DoschGuido Beldi
Jan 9, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Damiano BurattoFabio Mammano
Dec 22, 2020·Seizure : the Journal of the British Epilepsy Association·Ting-Ting YangFeng-Ru Tang
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia
Feb 3, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yuanfeng ZhuXin Liu
Jan 2, 2021·International Journal of Molecular Sciences·Katarina AndelovaNarcis Tribulova
Feb 15, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Shlok JindalGopinath Packirisamy
Apr 4, 2021·International Journal of Molecular Sciences·Jorge González-CasanovaDiana Rojas-Gomez
Jun 3, 2021·Journal of Cardiovascular Development and Disease·Spencer R MarshRobert G Gourdie
Jul 3, 2021·International Journal of Molecular Sciences·Erin M O'ShaughnessyPatricia E Martin
Aug 28, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Andrés Tittarelli

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Methods Mentioned

BETA
dissection

Software Mentioned

cellSens
SPSS Statistics
JM2

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